Abstract

Two Co(II)-containing coordination polymers, {Co(TDC)(TPB)_0.5·H₂O}_n (1) and {[Co₂(2,6-NDC)₂(TPB)]·MeCN·3H₂O}_n (2) (TPB = 1,2,4,5-tetra(4-pyridyl)benzene, H₂TDC = 2,5-thiophenedicarboxylic acid, 2,6-H₂NDC = 2,6-naphthalic acid), have been synthesized by a semi-rigid tetrapyridine ligand and auxiliary dicarboxylic ligands. The treatment ability of the compounds on skin necrosis after fracture was assessed and the related mechanism was discussed. First of all, the enzyme-linked immune sorbent assay (ELISA) detection kit was used in this research to detect the content level of the Recombinant Human Macrophage Colony stimulating Factor (MCSF) and Tumor Necrosis Factor-α (TNF-α) after compound treatment. Next, the real time quantitative polymerase chain reaction (RT-PCR) was conducted for the MMP2 and TIMP1 relative expression levels measurement.

摘要

两种含Co（II）的配位聚合物{Co（TDC）（TPB）_0.5 ·H ₂ O} _n（1）和{[Co ₂（2,6-NDC）₂（TPB）]·MeCN·3H ₂ O} _n（2）（TPB = 1,2,4,5-四（4-吡啶基）苯，H ₂ TDC = 2,5-噻吩二甲酸，2,6-H ₂NDC = 2,6-萘甲酸），是由半刚性四吡啶配体和辅助二羧酸配体合成的。评估了这些化合物对骨折后皮肤坏死的治疗能力，并探讨了相关机理。首先，本研究采用酶联免疫吸附试验（ELISA）检测试剂盒，检测复合后重组人巨噬细胞集落刺激因子（MCSF）和肿瘤坏死因子-α（TNF-α）的含量。治疗。接下来，进行实时定量聚合酶链反应（RT-PCR）以测量MMP2和TIMP1相对表达水平。