Abstract

Green nanotechnology, uses the extract of plants, microorganisms such as bacteria and fungi and its bioactive compounds for the synthesis of nanoparticles. This research work was focused on synthesis of Endophytic fungal silver nanoparticles (EFNps) from endophytic fungi Lasiodiplodia theobromae isolated from Cinnamomum zeylanicum. The aqueous extract of the endophytic fungi was used for the synthesis of silver nanoparticles and characterized by UV-Vis spectrophotometer, FT-IR spectroscopy, FESEM, Zeta Potential analysis and Particle size analysis (PSA). In order to validate the efficacy of EFNps, antimicrobial assay such as agar well diffusion method, Time kill curve, Minimum inhibitory concentration (MIC), Minimum bactericidal concentration (MBC), antibiofilm assays and pyocyanin assay were performed against P.aeruginosa ATCC (27853) and antibiotic resistant clinical strain. It was evident from the current study that EFNps could be the potential alternative to antibiotics to treat the infections caused by P. aeruginosa.

绿色纳米技术利用植物，细菌和真菌等微生物及其生物活性化合物的提取物来合成纳米颗粒。这项研究工作的重点是从分离自玉米肉桂的内生真菌Lasiodiplodia theobromae合成内生真菌银纳米颗粒（EFNps）。内生真菌的水提取物用于合成银纳米颗粒，并通过紫外可见分光光度计，傅立叶变换红外光谱，FESEM，Zeta电位分析和粒度分析（PSA）进行表征。为了验证EFNps的功效，对P进行了抗菌测定，例如琼脂井扩散法，时间杀灭曲线，最小抑菌浓度（MIC），最小杀菌浓度（MBC），抗生物膜测定和绿脓素测定。铜绿ATCC（27853）和抗生素耐药性临床菌株。从当前的研究中可以明显看出，EFNps可以替代抗生素来治疗由铜绿假单胞菌引起的感染。