ABSTRACT

The current study aims to assess the aqueous extract of Pinus wallichiana stem for the synthesis of small spherical-shaped (10–30 nm) silver nanoparticles (AgNPs) and their in-vitro and in-vivo biomedical applications. The biosynthesized AgNPs were nonmutagenic and safe at all test doses as per Ames and acute toxicity assay (20, 40, 60, and 80 mg/kg). The percent writhing inhibitory effect generated by AgNPs was 42.51, 50.84, and 59.06 at test doses of 10, 20, and 30 mg/kg, respectively. The percent decreased in gastrointestinal tract motility observed was 41.34%, 32.69%, and 28.48% at 10, 20, and 30 mg/kg, respectively. They also showed a significant antipyretic effect after 1, 2, and 3 h in comparison to normal saline. The AgNPs of P. wallichiana showed good antibacterial activity against Acinetobacter baumannii (60% with MIC₅₀ = 2.36 mg/ml and MBC = 5.0 mg/ml). These nanoparticles also possessed good antioxidant activity of 61.77 ± 0.828% and 70.25 ± 0.56% at 400 and 500 µg/ml, respectively and lack phytoagglutinin potential. Because of their high potency as biomedical agents, these nanoparticles can be a good alternative to the currently available drugs and approaches.

摘要

当前的研究旨在评估华山松茎的水提物，用于合成小的球形（10–30 nm）银纳米颗粒（AgNPs）及其在体外和体内的生物医学应用。根据Ames和急性毒性试验（20、40、60和80 mg / kg），在所有测试剂量下，生物合成的AgNPs都是非致突变性和安全的。在10、20和30 mg / kg的测试剂量下，AgNPs产生的扭曲抑制百分比分别为42.51、50.84和59.06。在10、20和30 mg / kg时，观察到的胃肠道运动力降低的百分比分别为41.34％，32.69％和28.48％。与生理盐水相比，它们在1、2和3小时后也显示出明显的解热作用。的AgNPP. wallichiana对鲍曼不动杆菌显示出良好的抗菌活性（60％，MIC ₅₀  = 2.36 mg / ml，MBC = 5.0 mg / ml）。这些纳米粒子在400和500 µg / ml时分别具有61.77±0.828％和70.25±0.56％的良好抗氧化活性，并且缺乏植物凝集素的潜力。由于它们作为生物医学药物的高效性，这些纳米颗粒可以替代当前可用的药物和方法。