Many researchers today are looking for mechanisms underlying plant defenses against nematodes by identifying differentially expressed genes in domesticated hosts. In order to provide a different perspective, we analyzed the root transcriptome of an undomesticated non‐host species, Solanum sisymbriifolium Lamark (SSI) before and after Globodera pallida infection. Utilizing RNAseq analyses, we identified changes in the expression of 277 transcripts. Many of these genes were not annotated; however, the annotated set included peroxidases, reactive oxygen species‐producing proteins, and regulators of cell death. Importantly, 60% of the nematode‐responsive genes did not respond to physical damage to root tissues, or to exogenous treatments with either salicylic acid or methyl jasmonate. Based on this, we speculate that the majority of changes in SSI gene expression were promoted by either nematode effectors, pathogen‐associated molecular patterns (PAMPs), or by exposure to untested endogenous signaling molecules such as ethylene, or by exposure to multiple stimuli. This study incorporates our findings into a model that accounts for part of this plant's unusual resistance to nematodes.

中文翻译：

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诱集农杆菌Solanum sisymbriifolium对Pallida pallida入侵的初步反应

如今，许多研究人员正在通过鉴定驯化宿主中差异表达的基因来寻找潜在的植物防御线虫的机制。为了提供不同的角度，我们分析了未驯化非宿主物种，根转录蒜芥茄前后Lamark（SSI）马铃薯金淡紫感染。利用RNAseq分析，我们确定了277个转录本表达的变化。这些基因中有许多没有注释。但是，带注释的集合包括过氧化物酶，产生活性氧的蛋白质和细胞死亡的调节剂。重要的是，线虫反应基因中有60％对根部组织的物理损伤或水杨酸或茉莉酸甲酯的外源处理均无反应。基于此，我们推测线虫效应子，病原体相关分子模式（PAMPs）或暴露于未经测试的内源性信号分子（如乙烯）或暴露于多种刺激都促进了SSI基因表达的大多数变化。这项研究将我们的发现整合到一个模型中，该模型解释了该植物对线虫的异常抗性。