Fusarium langsethiae is a symptomless pathogen of oat panicles that produces T‐2 and HT‐2 mycotoxins, two of the most potent trichothecenes produced by Fusarium fungi in cereals. In the last few years, the levels of these mycotoxin in oat grain has increased and the European commission have already recommended a maximum level for of 1000 μg kg⁻¹ for unprocessed oat for human consumption. The optimal and most sustainable way of combating infection and mycotoxin contamination is by releasing resistant oat varieties. Here the objective was to determine if we could identify any genomic loci associated with either the accumulation of F. langsethiae DNA or mycotoxins in the grain. In each of two years, field trials were conducted wherein 190 spring oat varieties were inoculated with a mixture of three isolate of the pathogen. Mycotoxins were quantified using liquid chromatography–tandem mass spectrometry. Varieties were genotyped using 16,863 genotyping by sequencing markers. Genome‐wide association studies associated 5 SNPs in the linkage group Mr06 with T‐2 + HT‐2 mycotoxin accumulation. Markers were highly correlated, and a single QTL was identified. The marker avgbs_6K_95238.1 mapped within genes showing similarity to lipase, lipase‐like or lipase precursor mRNA sequences and zinc‐finger proteins. These regions have previously been shown to confer a significant increase in resistance to Fusarium species.

中文翻译：

---

燕麦镰刀菌感染与真菌毒素积累的全基因组关联图谱（Avena sativa L.）

枯萎病镰刀菌是燕麦穗的一种无症状病原体，可产生T-2和HT-2霉菌毒素，这是谷类镰刀菌真菌产生的两种最强的毛霉菌毒素。在过去的几年中，燕麦谷物中这些霉菌毒素的含量增加了，欧洲委员会已经建议人类食用的未加工燕麦最高含量为1000μgkg ^-1。对抗感染和霉菌毒素污染的最佳，最可持续的方法是释放抗性燕麦品种。此处的目的是确定我们是否可以识别与浮游镰刀菌的积累相关的任何基因组位点谷物中的DNA或霉菌毒素。在两年的每一年中，进行了田间试验，其中用三种病原体的混合物接种了190个春燕麦品种。使用液相色谱-串联质谱法对霉菌毒素进行定量。使用16,863基因分型通过测序标记对品种进行基因分型。全基因组关联研究将Mr06连锁群中的5个SNP与T-2 + HT-2霉菌毒素的积累相关联。标记高度相关，并且鉴定出单个QTL。标记avgbs_6K_95238.1映射到与脂肪酶，类脂肪酶或脂肪酶前体mRNA序列和锌指蛋白相似的基因内。先前已证明这些区域赋予了对镰刀菌种的抗性的显着提高。