Endothelial cells are specialized epithelium lining the interior surface of vessels and play fundamental roles in angiogenesis, vascular permeability, and immune response. To identify endothelial cells in vivo, we constructed a Pecam1^{nlacZ‐H2B‐GFP/+} knock‐in mouse model in which the endothelial cells are labeled by nuclear LacZ (nlacZ) expression. When Pecam1^{nlacZ‐H2B‐GFP/+} mice are bred with germline Cre deleter mice, Pecam1^H2B‐GFP/+ line is created with native nuclear GFP (H2B‐GFP) expression in the endothelium of various organs. This dual reporter mouse provides us with a powerful genetic tool for definitive identification of endothelial cells and monitoring this important cell population throughout development, homeostasis, and disease conditions in mammals.

中文翻译：

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Pecam1 内皮特异性双报告小鼠模型的生成。

内皮细胞是衬在血管内表面的特化上皮细胞，在血管生成、血管通透性和免疫反应中起重要作用。为了在体内鉴定内皮细胞，我们构建了Pecam1 ^{nlacZ-H2B-GFP/+}敲入小鼠模型，其中内皮细胞被核 LacZ (nlacZ) 表达标记。当Pecam1 ^{nlacZ-H2B-GFP/+}小鼠与生殖系 Cre缺失小鼠繁殖时，Pecam1 ^H2B-GFP/+细胞系是在各种器官的内皮中用天然核 GFP (H2B-GFP) 表达创建的。这种双报告小鼠为我们提供了一种强大的遗传工具，用于确定内皮细胞的鉴定，并在哺乳动物的整个发育、稳态和疾病状况中监测这一重要细胞群。