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Kikuyugrass germplasm collections in the United States and Australia show low levels of genetic diversity as revealed by DArTseq genotyping
Crop Science ( IF 2.0 ) Pub Date : 2020-08-16 , DOI: 10.1002/csc2.20231
Marta Pudzianowska 1 , Tyler J. Mock 1 , Peter M. Martin 2 , Adam J. Lukaszewski 1 , James H. Baird 1
Affiliation  

Kikuyugrass [Cenchrus clandestinus (Hochst. ex Chiov.) Morrone (= Pennisetum clandestinum Hochst. ex Chiov.)] is a warm‐season grass native to Africa. It was introduced into the United States as forage in Hawaii and for erosion control in California. Kikuyugrass is considered invasive and currently is on the USDA's noxious weed list. Since complete eradication is difficult, it has become the primary species on several golf courses, athletic fields, and lawns. Kikuyugrass possesses exceptional quality with considerable cultural inputs, and little or no winter dormancy compared with other warm‐season turfgrasses. With breeding efforts directed specifically at reducing aggressiveness and improving texture, thus reducing inputs, it could become a valuable turf‐type species in coastal and inland California. The genetic diversity of kikuyugrass was investigated using single nucleotide polymorphism (SNP) and silicoDArT (presence or absence) markers revealed by the Diversity Arrays Technology sequencing (DArTseq) platform. Accessions were sampled throughout California, Hawaii, and Australia, both from natural stands and various collections. Among the 254 accessions tested, two distinct groups were discovered, and there was no geographic pattern to this differentiation. The overall level of SNP polymorphism was low (polymorphic information content [PIC] average = .33, PIC median = .38). Most (76%) of the observed genetic variation was within populations, whereas 24% was among populations. Average genetic distances within populations ranged from 0.09 to 0.16, whereas distances among populations ranged from 0.13 to 0.36. Accessions from Hawaii and Australia were the most diverse; however, a detectable level of genetic diversity of kikuyugrass also exists in California, mostly because of the past introductions from Australia.

中文翻译:

DArTseq基因分型表明,美国和澳大利亚的菊苣菜种质资源显示出较低的遗传多样性

Kikuyugrass [ Cenchrus clandestinus(Hochst。ex Chiov。)Morrone(= 狼尾草clandestinum赫斯特 原属Chiov。)]是非洲原产的暖季草。它在夏威夷作为草料传入美国,并在加利福尼亚用于侵蚀控制。Kikuyugrass被认为是侵入性的,目前在USDA的有害杂草清单中。由于很难彻底根除,因此它已成为数个高尔夫球场,运动场和草坪上的主要物种。与其他暖季型草皮草相比,基库尤格拉斯草具有非凡的品质和可观的文化投入,并且冬季休眠很少甚至没有。通过专门针对减少侵略性和改善质地,从而减少投入的育种努力,它可能成为加利福尼亚沿海和内陆的一种有价值的草皮型物种。使用单核苷酸多态性(SNP)和silicoDArT(存在或不存在)标记物研究了菊苣的遗传多样性,该标记物通过多样性阵列技术测序(DArTseq)平台揭示。收集的样品来自加利福尼亚州,夏威夷和澳大利亚,分别来自自然林和各种收藏。在测试的254个种质中,发现了两个不同的组,并且没有这种分化的地理模式。SNP多态性的总体水平较低(多态信息含量[PIC]平均= .33,PIC中位数= .38)。观察到的大多数遗传变异(76%)在种群内,而24%在种群间。群体内的平均遗传距离为0.09至0.16,而群体间的平均遗传距离为0.13至0.36。夏威夷和澳大利亚的种质最多。然而,在加利福尼亚州,也存在可检测水平的菊苣苔的遗传多样性,这主要是由于澳大利亚过去的引进。
更新日期:2020-08-16
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