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DNA content and ploidy estimation of Festuca ovina accessions by flow cytometry
Crop Science ( IF 2.0 ) Pub Date : 2020-08-10 , DOI: 10.1002/csc2.20229
Yinjie Qiu 1, 2 , Sierra Hamernick 1 , Joan Barreto Ortiz 1 , Eric Watkins 1
Affiliation  

Festuca ovina L. is a fine fescue that is used as a low‐input turfgrass. The ploidy levels of F. ovina accessions held by the USDA National Plant Germplasm System (NPGS) are unknown, limiting the use of the germplasm in breeding programs. The objective of this study was to determine DNA content and estimate ploidy of these accessions. Among 127 accessions, we identified a wide range of ploidy levels from diploid to octoploid. We also found the accessions with higher ploidy levels usually had larger seed size. These results will be informative to plant breeders and researchers using germplasm from the F. ovina collection and point to challenges in maintaining polyploid, outcrossing germplasm seed stocks in common nurseries.

中文翻译:

流式细胞术分析羊茅卵的DNA含量和倍性估计。

Festuca ovina L.是一种优良的羊茅,用作低投入的草皮草。F的倍性水平。美国农业部国家植物种质资源系统(植物种质资源系统)举行种质是未知的,限制了育种计划利用种质资源。这项研究的目的是确定DNA含量并估计这些种的倍性。在127个种质中,我们鉴定了从二倍体到八倍体的广泛倍性水平。我们还发现具有较高倍性水平的种质通常具有较大的种子大小。这些结果将为植物育种者和研究人员提供有用的信息,这些研究人员使用了来自F. ovina采集的种质,并指出了维持多倍体,在普通苗圃中越过种质种子库的挑战。
更新日期:2020-08-10
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