1698

Harnessing synthetic biology to enhance heterologous protein expression

Shlomo Zarzhitsky, Alex Jiang, Elizabeth E. Stanley, and Michael H. Hecht

Expressing heterologous proteins in microbial hosts is a core technology for protein science. However, heterologous protein expression often produces low yields and/or misfolded products. To overcome these challenges, a protein of interest (POI) can be joined to another protein that endows the fusion with favorable properties for expression and/or purification. All the currently used fusion partners are derived from natural sequences that evolved for other purposes. Here we demonstrate that novel proteins, which did not evolve in nature, can serve as fusion partners; and these De novo Expression Enhancer Proteins (DEEPs) facilitate high level expression and facile purification of a range of heterologous proteins and peptides.https://onlinelibrary.wiley.com/doi/10.1002/pro.3907

中文翻译：

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在这个问题上

1698

利用合成生物学增强异源蛋白表达

Shlomo Zarzhitsky，Alex Jiang，Elizabeth E.Stanley和Michael H.Hecht

在微生物宿主中表达异源蛋白质是蛋白质科学的一项核心技术。但是，异源蛋白表达通常产生低产量和/或错误折叠的产物。为了克服这些挑战，可以将目的蛋白（POI）与另一种蛋白结合，使融合蛋白具有用于表达和/或纯化的有利特性。当前所有使用的融合伴侣均源自为其他目的而进化的天然序列。在这里，我们证明了自然界中不会进化的新型蛋白质可以作为融合伴侣。这些从头表达增强蛋白（DEEP）促进了一系列异源蛋白和肽的高水平表达和便捷纯化.https：//onlinelibrary.wiley.com/doi/10.1002/pro.3907