Abstract In this work, we evaluate the performance of two biofunctionalization processes on silicon and hydrogenated amorphous silicon carbide (a-SiC:H). The biofunctionalization processes were designed to immobilize antibodies via non-specific physical adsorption or covalent attachment. The impact of the two surface types (crystalline and amorphous) on the resulting immunosensing layer is discussed in terms of the possible orientation, stability, and bioactivity of the immobilized antibodies. To evaluate the formation of active groups on the surface before and after the immobilization process, we used Fourier-transform infrared (FTIR) spectroscopy. On the other hand, to visualize the topography changes on the different surfaces with immobilized antibodies, we used atomic force microscopy (AFM). ELISA assay was conducted to obtain a quantitative parameter associated with the density of immobilized antibodies on the platforms. The results showed that the antibodies were immobilized on both platforms by any of the two immobilization mechanisms. The antigen capture did not show a direct relationship with the antibody estimation made by ELISA. According to the results, the a-SiC:H platforms by covalent attachment achieved the highest density of immobilized antibodies compared to silicon. However, its performance in the antigen detection assay was lower compared to silicon platforms. We concluded that the performance of the silicon platform was better in terms of its biofunctionalization and antigen detection. The orientation and structural integrity of the antibodies on the platforms was crucial to its performance on antigen detection.

中文翻译：

---

硅和氢化非晶碳化硅作为免疫传感器的生物功能平台

摘要 在这项工作中，我们评估了两种生物功能化工艺对硅和氢化非晶碳化硅 (a-SiC:H) 的性能。生物功能化过程旨在通过非特异性物理吸附或共价连接固定抗体。从固定抗体的可能取向、稳定性和生物活性方面讨论了两种表面类型（结晶和无定形）对所得免疫传感层的影响。为了评估固定过程前后表面活性基团的形成，我们使用了傅里叶变换红外 (FTIR) 光谱。另一方面，为了用固定化抗体可视化不同表面的形貌变化，我们使用了原子力显微镜 (AFM)。进行ELISA测定以获得与平台上固定抗体密度相关的定量参数。结果表明，抗体通过两种固定机制中的任何一种固定在两个平台上。抗原捕获未显示与通过 ELISA 进行的抗体估计有直接关系。根据结果​​，与硅相比，通过共价连接的 a-SiC:H 平台实现了最高密度的固定化抗体。然而，与硅平台相比，其在抗原检测测定中的性能较低。我们得出结论，硅平台在生物功能化和抗原检测方面的性能更好。平台上抗体的方向和结构完整性对其抗原检测性能至关重要。