Abstract The innovative aspect of nanoparticles takes on an important role in the advancement of research on nanostructured materials. The objective of the present study was to carry out an investigation of the interactions between lactase enzyme and carbon nanotubes applied to the elaboration of a chemically modified carbon paste electrode. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), fluorescence spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric (TG/DTG) were conducted to comprehend the interactions and the efficiency of the enzyme immobilization in carbon nanotubes. Through studying the morphology of the materials using SEM and TEM, it was shown that there was adsorption of the lactase enzyme into the tubular area of the carbon nanotubes. Through fluorescence spectroscopy analysis, it was observed that the emission of lactase fluorescence is mainly due to tryptophan residue (Trp), and this fluorescence was reduced in the presence of CNT, demonstrating the interaction between these components. In the remaining analyses, FTIR, XRD and TG/DTG, adsorption of the lactase enzyme into the carbon nanotube matrix was demonstrated.

中文翻译：

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固定在碳纳米管基质中的乳糖酶相互作用的研究，用于开发化学修饰的碳糊电极

摘要 纳米粒子的创新性在推动纳米结构材料研究方面发挥着重要作用。本研究的目的是对乳糖酶和碳纳米管之间的相互作用进行研究，该碳纳米管用于制备化学改性碳糊电极。进行扫描电子显微镜 (SEM)、透射电子显微镜 (TEM)、荧光光谱、傅里叶变换红外光谱 (FTIR)、X 射线衍射 (XRD) 和热重 (TG/DTG) 以了解相互作用和效率酶在碳纳米管中的固定化。通过使用 SEM 和 TEM 研究材料的形态，表明乳糖酶吸附到碳纳米管的管状区域。通过荧光光谱分析，观察到乳糖酶荧光的发射主要是由色氨酸残基 (Trp) 引起的，在 CNT 存在下这种荧光减弱，表明这些成分之间存在相互作用。在其余的分析中，FTIR、XRD 和 TG/DTG 证明了乳糖酶吸附到碳纳米管基质中。