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Nematophagous and endomycorrhizal fungi in the control of Meloidogyne incognita in soybean
Rhizosphere ( IF 3.4 ) Pub Date : 2020-06-17 , DOI: 10.1016/j.rhisph.2020.100222
Vinícius Rigueiro Messa , Antônio Carlos Torres da Costa , Odair José Kuhn , Camila Torres Stroze

The root knot nematode (Meloidogyne spp.) is a widely distributed phytoparasite and causes production losses in soybeans. Therefore, the aim of the present study was to evaluate the isolates of Purpureocillium lilacinum (Simbiose®), Claroideoglomus etunicatum and Rhizophagus clarus as possible biocontrol agents for Meloidogyne incognita. The greenhouse treatments consisted of six distinct formulations of fungal isolates: T1 - Witness; T2 - P. lilacinum (100 μl−1); T3 - C. etunicatum (23.5 mL−1); T4 - R. clarus (23.5 mL−1); T5 - P. lilacinum (100 μl−1) + C. etunicatum (23.5 mL−1); T6 - P. lilacinum (100 μl−1) + R. clarus (23.5 mL−1) with five repetitions each. The nematophagous fungus was applied in liquid suspension and the inoculums of arbuscular mycorrhizal fungi (AMFs) composed of propagules (spores, hyphae, colonized root fragments) in the sowing furrow. The sowing of two soybean seeds susceptible to M. incognita was carried out in 2L pots with a mixture of sand and earth in proportion (3:1). The inoculation of 2000 eggs and eventual juveniles of the second stage (J2) of M. incognita per plant was carried out in sequence. Sixty-five days after inoculation, the plants were removed to evaluate the vegetative, mycorrhizal and nematological variables. The data were submitted to a media comparison test with a 5% probability of error. The biocontrol potential of these fungi becomes an agricultural alternative for the biological management of Meloidogyne spp., as well as for the sustainability of agriculture and biological and biotechnological production.



中文翻译:

线虫和内生菌根真菌控制大豆根结线虫

根结线虫(Meloidogyne spp。)是一种分布广泛的植物寄生虫,会导致大豆生产损失。因此,本研究的目的是评估的株Purpureocillium lilacinum(Simbiose®),Claroideoglomus etunicatumRhizophagus CLARUS作为用于可能的生物防治剂南方根结线虫。温室处理由六种不同的真菌分离物组成:T1-见证;T2-丁香假单胞菌(100μl -1); T3-牛膝草(23.5mL -1);T4-克拉斯菌(23.5mL -1);T5 - P. lilacinum(100μl -1)+ 棉衣梭菌(23.5 mL -1); T6 - P. lilacinum(100微升-1)+  R. CLARUS(23.5毫升-1)与每个重复五次。将线虫真菌应用于液体悬浮液中,并在播种犁沟中接种由繁殖体(孢子,菌丝,定植的根碎片)组成的丛枝菌根真菌(AMF)。在2L盆中按比例(3:1)的沙子和泥土播种2种易受隐孢子虫感染的大豆种子。的2000个鸡蛋和第二阶段(J2)的最终青少年接种南方根结线虫每个植物按顺序进行。接种后六十五天,将植物移出以评估营养,菌根和线虫学变量。数据以5%的错误概率提交给媒体比较测试。这些真菌的生物防治潜力已成为农业上对根结线虫的生物管理以及农业和生物技术生产的可持续性的替代品。

更新日期:2020-06-17
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