Soil salinity, the most critical environmental stress hampering crop productivity in drier agro-ecosystems, is mitigated through various management strategies. A preference for a non-chemical, eco-friendly, and economical approach has led to the investigation of fluorescent pseudomonads, a common group of plant growth-promoting rhizobacteria (PGPR), known to alleviate stress. In the present study, salinity-sensitive finger millet (Indaf-9) seeds treated with 40 fluorescent pseudomonad isolates were obtained from different saline regions of India. From these, 20 were selected based on growth promotion studies. These isolates were again subjected to growth promotion studies but under varied saline concentrations (0–350 mM NaCl). The results obtained were validated with studies on growth kinetics under a gradient of NaCl concentrations. Three strains, SPF-5, SPF-33, SPF-37, were selected based on their performance and characterized. They were screened for their plant growth-promoting traits (indole acetic acid, biofilm, siderophore, and ACC deaminase), stress-related physiological traits (enzymatic antioxidants, free proline, H₂O₂, lipid peroxidation, total protein, phenolics, flavonoids, total chlorophyll, leaf relative water content, and dry weight) and growth parameters under green-house conditions. Under increased salinity stress, strain SPF-33 showed increased activity of enzymatic antioxidants and elevation in proline content along with decreased lipid peroxidation and H₂O₂, with increased plant height and number of spikelets. Similarly, treatment with SPF-37 exhibited a significant increase in germination, vigor index, increased plant height, and the number of spikelets, total chlorophyll, phenolics, flavonoids, proteins, and leaf relative water content under 350 mM NaCl as compared to the control. Overall, our results indicate the fluorescent Pseudomonad strains SPF-33 and SPF-37 are strong candidates for alleviating salt stress in the field.

土壤盐分是干旱农业生态系统中阻碍作物生产力的最关键的环境压力，可通过各种管理策略来缓解。对非化学，环保和经济方法的偏爱导致对荧光假单胞菌的研究，荧光假单胞菌是一组常见的植物促生根瘤菌（PGPR），已知可减轻压力。在本研究中，从印度的不同盐碱地区获得了用40种荧光假单胞菌分离物处理的盐度敏感性小米（Indaf-9）种子。从这些中，基于促进生长的研究选择了20个。这些分离株再次进行了促进生长的研究，但在不同的盐浓度（0–350 mM NaCl）下进行。通过在NaCl浓度梯度下的生长动力学研究验证了所获得的结果。三株 根据其性能和特性选择了SPF-5，SPF-33，SPF-37。筛选了他们的植物生长促进性状（吲哚乙酸，生物膜，铁载体和ACC脱氨酶），与胁迫相关的生理性状（酶促抗氧化剂，游离脯氨酸，H₂ O ₂，脂质过氧化，总蛋白，酚类，类黄酮，总叶绿素，叶片相对水含量和干重）和温室条件下的生长参数。在盐度胁迫下，菌株SPF-33的酶促抗氧化剂活性增强，脯氨酸含量升高，脂质过氧化和H ₂ O _2降低，株高和小穗数量增加。同样，与对照组相比，用SPF-37处理在350 mM NaCl下显示出发芽，活力指数，植物高度增加，小穗数，总叶绿素，酚，类黄酮，蛋白质和叶片相对含水量显着增加。 。总的来说，我们的结果表明荧光假单胞菌菌株SPF-33和SPF-37是缓解田间盐胁迫的强候选者。