The use of porous silicon (PS) for biosensing applications is now emerging as it offers desired properties such as better surface-volume ratio, tunable pore size, ease of fabrication, and compatibility with the current silicon-based technology. These superior properties make PS as a promising candidate for the immobilization of biomolecules with higher retention, stability, storage, and operational ease. High specificity and catalytic activity of the enzyme for the substrate are advantageous for the detection of a particular analyte with higher accuracy in less time. Immobilization of enzyme on the PS surface alters the surface electrical and optical properties, and can conveniently be used for the sample analysis. In the present review, the strategies of enzyme immobilization on PS and various influencing factors are discussed in detail. The scope of PS for enzyme immobilization and prerequisite surface modifications has been assessed in brief. The review discusses the various methods of enzyme immobilization on PS with their benefits and bottlenecks with special emphasis on the approaches to overcome the difficulties during the immobilization process. Further, various properties of PS influencing the process of immobilization have been outlined in brief. An overall analysis of enzyme immobilization on PS surface has been given for the successful development of PS based enzymatic biosensors.

多孔硅（PS）在生物传感应用中的使用正在出现，因为它提供了所需的特性，例如更好的表面体积比，可调的孔径，易于制造以及与当前基于硅的技术的兼容性。这些优越的性能使PS成为具有更高保留，稳定性，储存性和操作简便性的生物分子固定化的有前途的候选者。酶对底物的高特异性和催化活性有利于在较短的时间内以较高的准确度检测特定的分析物。将酶固定在PS表面会改变表面的电学和光学特性，可方便地用于样品分析。在本综述中，详细讨论了将酶固定在PS上的策略和各种影响因素。简要评估了用于酶固定和必要的表面修饰的PS的范围。这篇综述讨论了将酶固定在PS上的各种方法的优点和瓶颈，特别强调了克服固定过程中困难的方法。此外，已简要概述了影响固定过程的PS的各种特性。为了成功开发基于PS的酶生物传感器，已对PS表面固定化酶进行了全面分析。这篇综述讨论了将酶固定在PS上的各种方法的优点和瓶颈，特别强调了克服固定过程中困难的方法。此外，已简要概述了影响固定过程的PS的各种特性。为了成功开发基于PS的酶生物传感器，已对PS表面固定化酶进行了全面分析。这篇综述讨论了将酶固定在PS上的各种方法的优点和瓶颈，特别强调了克服固定过程中困难的方法。此外，已简要概述了影响固定过程的PS的各种特性。为了成功开发基于PS的酶生物传感器，已对PS表面固定化酶进行了全面分析。