A two-year field experiment was carried out in order to study the occurrence degree and mechanism of apple replant disease (ARD) in the apple orchards with different soil textures. So we can adopt appropriate control measures according to the severity of ARD. Healthy two-year-old seedlings with consistent growth were selected, of which the root stock was T337 and the scion was Yanfu 3. There were significant differences in biomass between methyl bromide fumigation and replanted treatments, and the difference was the largest in clay loam, followed by sandy loam, and loam, which verified ARD in clay loam was most serious, followed by sandy loam and loam. Based on high-throughput sequencing of fungi in soil samples, fungal richness and diversity were the highest in clay loam, followed by sandy loam, and loam. The relative abundance of Fusarium in SX, SL, FX, FL, WX and WL was 7.33%, 19.32%, 2.70%, 4.24%, 10.71% and 23.87%, respectively. Based on Real-time quantitative analysis, there were significant differences in the number of Fusarium oxysporum and Fusarium solani between methyl bromide fumigation and replanted treatments, i.e., clay loam > sandy loam > loam. Fusarium was the main pathogen causing ARD. This shows that ARD is the most serious under replanted clay loam condition. High-throughput sequencing technology was used to prove the difference in Fusarium was one of the important reasons for ARD under different soil textures. This technology provides a new idea for the prevention and control of ARD.

为了研究土壤质地不同的苹果园中苹果再植病（ARD）的发生程度和机理，进行了为期两年的田间试验。因此，我们可以根据ARD的严重程度采取适当的控制措施。选择健康的两年生苗，苗木生长一致，其中根茎为T337，接穗为盐阜3。甲基溴熏蒸和再处理之间生物量差异显着，其中黏土壤土差异最大。 ，其次是沙壤土和壤土，这证明粘土壤土中的ARD最严重，其次是沙壤土和壤土。根据土壤样品中真菌的高通量测序，真菌的丰富度和多样性在黏土壤土中最高，其次是沙壤土和壤土。相对丰度SX，SL，FX，FL，WX和WL中的镰刀菌分别为7.33％，19.32％，2.70％，4.24％，10.71％和23.87％。根据实时定量分析，熏蒸和补植处理之间的尖孢镰刀菌和茄形镰刀菌的数量存在显着差异，即粘土壤土>沙质壤土>壤土。镰刀菌是引起ARD的主要病原体。这表明在补植的壤土条件下，ARD是最严重的。利用高通量测序技术来证明镰刀菌的差异是土壤质地不同引起ARD的重要原因之一。该技术为预防和控制ARD提供了新思路。