Paeonia species are important ornamental plants, including three types: tree peony, herbaceous peony, and Itoh hybrid peony. Paeonia with yellow color is desired and becoming popular. However, the chemical mechanism of the formation of yellow flowers in Paeonia is still unclear. In this study, 14 representative samples were selected: four samples of three species of Paeonia, four herbaceous peony cultivars, three lutea hybrid tree peony cultivars, and three Itoh hybrid peony cultivars. The petal extracts of the samples were determined by HPLC-DAD and HPLC-Q-TOF-MS/MS. In total, 29 flavonoids were isolated and identified, including 28 flavonol glycosides and one chalcone derivative. There were significant differences in the composition and content of flavonoids in petals of different cultivars (species). The total content of flavonoids was between 19.430 and 143.043 mg • g⁻¹ DW. Flavonol glycosides were detected in all samples. Chalconaringenin 2′-O-glucoside was detected from one herbaceous peony cultivar ‘Golden Wheel’ lutea hybrid tree peony cultivars, and Itoh peony cultivars. The content of chalconaringenin 2′-O-glucoside in the samples was more than 43% of total flavonoids. The pigment type and content of lutea hybrid tree peonies and Itoh hybrid peonies are similar. Total flavonoids, especially quercetin 3-O-galloylglucoside and chalconaringenin 2′-O-glucoside contribute greatly to the formation of yellow flowers of Paeonia. The results of this study provide a comprehensive understanding of the chemical mechanism for yellow flower coloration of Paeonia, and lay the foundation for yellow flower breeding of Paeonia.

e药是重要的观赏植物，包括三种类型：牡丹，草本牡丹和伊藤杂种牡丹。带有黄色的药是人们所希望的并且变得流行。然而，在Pa药中形成黄色花的化学机制仍不清楚。在这项研究中，选择了14个代表性样品：三种Pa药的四个样品，4个草本牡丹品种，3个lutea杂交树牡丹品种和3个Itoh杂交牡丹品种。通过HPLC-DAD和HPLC-Q-TOF-MS / MS测定样品的花瓣提取物。总共分离并鉴定了29种黄酮类化合物，包括28种黄酮醇苷和一种查尔酮衍生物。不同品种（品种）的花瓣中黄酮的成分和含量存在显着差异。类黄酮的总含量在19.430至143.043 mg•g ^-1 DW之间。在所有样品中均检测到黄酮醇苷。从一种草本牡丹品种“金轮”黄茶杂交牡丹品种和伊藤牡丹品种中检出查尔香豆素2'- O-葡萄糖苷。查尔冈丁烯酮2'- O的含量样品中的β-葡萄糖苷占总类黄酮的43％以上。绿茶杂种牡丹和伊藤杂种牡丹的色素类型和含量相似。总黄酮，槲皮素尤其3- ö -galloylglucoside和chalconaringenin 2'- ö葡糖苷大大有助于的黄色的花的形成芍药。这项研究的结果提供的化学机制的黄花着色全面了解芍药，并为黄花繁殖的基础芍药。