White rust caused by Puccinia horiana is a destructive disease of chrysanthemum plants. To better understand the resistance mechanisms of composite species to this disease, the leaf cuticular traits, antioxidant and defensive enzymes activities of immune (Chrysanthemum makinoi var. wakasaense) and highly susceptible (Ajania shiwogiku var. kinokuniense) species were compared. Trichome density of two species was markedly different, negatively associated with plant resistance to P. horiana. Total wax load in C. makinoi var. wakasaense was two times more than that in A. shiwogiku var. kinokuniense. The wax composition in immune one was abundant in esters and primary alcohols. Superoxide dismutase (SOD, EC 1.15.1.1), peroxidase (POD, EC 1.11.1.7), polyphenoloxidase (PPO, EC 1.14.18.1 or EC 1.10.3.2) and phenylalanine ammonia lyase (PAL, EC 4.3.1.5) activities were investigated. In C. makinoi var. wakasaense, the activity of SOD and POD increased rapidly after inoculation, which might be non-host induced reactive oxygen species (ROS) activated antioxidant enzymes, however SOD and POD remained a low and steady level in the highly susceptible one after inoculation. Quick increase in PPO activities after inoculation was observed in both species, however it remained higher in C. makinoi var. wakasaense at the late period of inoculation. PAL in C. makinoi var. wakasaense was induced after pathogen inoculation, but not in A. shiwogiku var. kinokuniense, suggesting that these two enzymes might contribute to the resistance to P. horiana.

由Puccinia horiana引起的白锈病是菊花植物的破坏性疾病。为了更好地理解复合种的抗性机制本病，叶表皮特征，抗氧化和免疫防御酶活性（菊花把先岛变种wakasaense）和高感（亚菊shiwogiku变种kinokuniense）的物种进行比较。两种物种的套茎密度显着不同，与植物对霍桑假单胞菌的抗性呈负相关。C. makinoi var。中的总蜡负荷。wakasaense是A. shiwogiku var中的两倍。kinokuniense。免疫蜡中的蜡成分富含酯和伯醇。研究了超氧化物歧化酶（SOD，EC 1.15.1.1），过氧化物酶（POD，EC 1.11.1.7），多酚氧化酶（PPO，EC 1.14.18.1或EC 1.10.3.2）和苯丙氨酸氨裂合酶（PAL，EC 4.3.1.5）的活性。在C. makinoi var。wakasaense，接种后SOD和POD的活性迅速增加，这可能是非宿主诱导的活性氧（ROS）激活的抗氧化酶，但是接种后高敏感人群中SOD和POD仍保持较低且稳定的水平。在两个物种中都观察到接种后PPO活性迅速增加，但是在C. makinoi var中仍然较高。若森在接种后期。C. makinoi var中的PAL 。wakasaense是在病原菌接种后诱导的，但不是在A. shiwogiku var中诱导的。kinokuniense，表明这两种酶可能有助于对霍氏疟原虫的抗性。