Among the economically important horticultural crops, citrus is one of the most vulnerable crops to soil salinity. Rangpur lime is more tolerant to high soil salinity than other commonly used citrus rootstocks. However, the molecular mechanism involved in salinity tolerance has not been explored in Rangpur lime. In this study, a cDNA library was constructed from leaves of Rangpur lime watered for 30 days with 100 mmol · L⁻¹ NaCl in tap water or tap water using suppression subtractive hybridization (SSH). Two hundred cDNA clones randomly selected from this library were sequenced, and an average of 569 bp was obtained from the majority of clones. Fifty-six salinity-induced genes, showing 2- to 6-fold increases in their expression levels, were identified by macroarray hybridizations. Salinity-induced genes were associated with transcription (5.36%), stress response and signaling (21.43%), metabolism (16.07%), transport facilitation (10.71%), photosynthesis (10.71%), protein synthesis and fate (19.64%) and cellular biogenesis (3.57%). Stress response- and signaling-related genes constituted the largest functional group, associated with the production of compatible solutes, regulation of stomatal movement, lipid modification, oxidative stress, antioxidant defense, and stress signaling. Expression levels of 13 identified genes were induced 1.8- to 3.1-fold, which were validated in salt-treated and untreated Rangpur lime. The functions of salinity-induced, stress-related genes and their potential roles in salinity tolerance in Rangpur lime were discussed.

在具有重要经济意义的园艺作物中，柑桔是最容易受到土壤盐分伤害的作物之一。朗布尔石灰比其他常用的柑橘砧木更耐高盐分。然而，在朗普尔石灰中尚未探索涉及耐盐性的分子机制。在这项研究中，使用抑制消减杂交法，从自来水或自来水中用100mmol·L ^-1 NaCl浇水30天的兰布尔青柠叶构建cDNA文库。对从该文库中随机选择的200个cDNA克隆进行测序，从大多数克隆中平均获得569 bp 。通过大阵列杂交鉴定出五十六个盐度诱导的基因，其表达水平提高了2至6倍。盐度诱导的基因与转录（5.36％），应激反应和信号（21.43％），新陈代谢（16.07％），运输促进（10.71％），光合作用（10.71％），蛋白质合成和命运（19.64％）相关。细胞生物发生率（3.57％）。与应激反应和信号相关的基因构成最大的功能组，与相容性溶质的产生，气孔运动的调节，脂质修饰，氧化应激，抗氧化防御和应激信号传导相关。13种已鉴定基因的表达水平被诱导了1.8到3.1倍，这在盐处理和未经处理的朗布尔石灰中得到了验证。盐度诱导的功能