Pectobacterium carotovorum is the causal agent of bacterial soft rot in a wide range of vegetable host species. Once P. carotovorum infects the plant, the spread of the disease is difficult to control. In this study, a rapid and sensitive method based on loop-mediated isothermal amplification (LAMP) was developed for detecting P. carotovorum in celery with soft rot using a primer set designed from the pmrA conserved sequence of P. carotovorum. The specificity of the LAMP primer set for P. carotovorum was extensively validated on both P. carotovorum strains and non-target strains. The sensitivity was 1 pg of P. carotovorum genomic DNA, which demonstrated 10 times more sensitive than the conventional PCR assay. LAMP was also used to detect P. carotovorum in bacterial suspension. The lowest detection concentration was 10⁴ CFU · mL⁻¹. In addition, a LAMP assay, in conjunction with a crude DNA extraction method, was successfully performed on P. carotovorum-infected samples derived from both artificially and naturally infected plants. In summary, the LAMP assay established in this study constitutes a simple, sensitive, and rapid method for the detection of P. carotovorum, and has potential application in the control of celery soft rot disease through early detection.

胡萝卜腐菌是许多植物宿主物种中细菌软腐的病因。一旦胡萝卜假单胞菌感染了植物，该病的传播就难以控制。在这项研究中，基于环介导等温扩增（LAMP）的快速和灵敏的方法是用于检测显影P. carotovorum使用的引物组从设计软腐病在芹菜PMRA保守的序列P. carotovorum。LAMP引物对胡萝卜假单胞菌的专一性已在胡萝卜假单胞菌菌株和非靶标菌株上得到了广泛验证。敏感度为1 pg的胡萝卜假单胞菌基因组DNA，其灵敏度是传统PCR测定法的10倍。LAMP还用于检测细菌悬浮液中的胡萝卜假单胞菌。最低检测浓度为10 ⁴ CFU·mL ^-1。另外，还成功地对衍生自人工和自然感染植物的胡萝卜腐菌感染的样品进行了LAMP测定，并结合了粗DNA提取方法。综上所述，本研究建立的LAMP测定法是一种简单，灵敏，快速的方法，用于检测胡萝卜假单胞菌，并通过早期检测在控制芹菜软腐病中具有潜在的应用价值。