Meiotic recombination and the resulting novel allele combinations are fundamental to plant breeding and the identification of superior hybrids. However, the rate of meiotic crossovers is naturally suppressed below its potential ability, which prevents plant breeding efficiency. Nine suppressors of meiotic recombination have been identified in the model plant Arabidopsis and in other crop species. Mutations in these genes can lead to increased recombination frequency and could therefore potentially be used to create hyper-recombinant lines for ornamental breeding. In Gerbera hybrida, the anti-crossover factors remain elusive. In this study, we isolated and cloned TOP3α from flower buds of G. hybrida, and it encoded 935 amino acids with three conserved domains TOPRIM, TOP1Ac and zf-GR. Moreover, TOP3α was the highest expressed at the flower bud stage, which coincided with the occurrence of meiotic recombination, suggesting that TOP3α is associated with the regulation of meiotic recombination in G. hybrida.

减数分裂重组和由此产生的新等位基因组合对于植物育种和鉴定优良杂种至关重要。但是，减数分裂交换的速率自然被抑制到其潜在能力以下，这阻止了植物的育种效率。在模型植物拟南芥和其他农作物中已经鉴定出九种减数分裂重组抑制剂。这些基因中的突变可导致重组频率增加，因此可能潜在地用于创建用于观赏育种的超重组品系。在非洲菊杂交种中，抗交叉因子仍然难以捉摸。在本研究中，我们从G. hybrida的花芽中分离并克隆了TOP3α 。，它编码935个氨基酸，具有三个保守域TOPRIM，TOP1Ac和zf-GR。此外，TOP3α在花芽期表达最高，这与减数分裂重组的发生相吻合，这表明TOP3α与G. hybrida中减数分裂重组的调控有关。