This study aimed to investigate the relationships between LPCAT1 expression and clinicopathologic parameters of hepatocellular carcinoma (HCC), further, to explore the effect of LPCAT1 on overall survival (OS) in patients with HCC, and its possible mechanism. Bioinformatics analysis using high throughput RNA-sequencing data from TCGA was utilized to explore the differential expression of LPCAT1 between normal and tumor tissues, and the associations between LPCAT1 expression and clinicopathological parameters. Survival analyses and subgroup survival analyses were utilized to elucidate the effect of LPCAT1 on OS in patients with HCC. Univariate analysis and multivariate analysis were used to investigate the prognostic factors. Potential LPCAT1 related tumor genes were identified by the methodology of differentially expressed genes (DEGs) screening. GO term enrichment analysis, KEGG pathway analysis and the PPI network were used to explore the potential mechanism. LPCAT1 was significantly overexpressed in HCC tumor tissues compared with normal tissues. The LPCAT1 expression was related to tumor grade, ECOG score, AFP and TNM stage, with P values of 0.000, 0.000, 0.007 and 0.000, respectively. Multivariate analysis demonstrated that LPCAT1 expression was independently associated with OS, with an HR of 1.04 (CI: 1.01–1.06, P = 0.003). The KEGG pathway enrichment analyses showed that overlapped DEGs mainly participate in the cell cycle. Finally, we identified a hub gene, CDK1, which has been reported to act on the cell cycle, consistent with the result of KEGG enrichment analysis. Collectively, these data confirmed LPCAT1 was upregulated in HCC, and was an independent predictor of the prognosis.

本研究旨在探讨LPCAT1表达与肝细胞癌（HCC）临床病理参数的关系，进一步探讨LPCAT1对HCC患者总生存期（OS）的影响及其可能机制。使用来自 TCGA 的高通量 RNA 测序数据的生物信息学分析用于探索 LPCAT1 在正常组织和肿瘤组织之间的差异表达，以及 LPCAT1 表达与临床病理学参数之间的关联。生存分析和亚组生存分析用于阐明 LPCAT1 对 HCC 患者 OS 的影响。采用单因素分析和多因素分析探讨预后因素。通过差异表达基因（DEGs）筛选的方法鉴定了潜在的LPCAT1相关肿瘤基因。GO术语富集分析、KEGG通路分析和PPI网络用于探索潜在机制。与正常组织相比，LPCAT1 在 HCC 肿瘤组织中显着过表达。LPCAT1 的表达与肿瘤分级、ECOG 评分、AFP 和 TNM 分期有关，具有P值分别为 0.000、0.000、0.007 和 0.000。多变量分析表明，LPCAT1 表达与 OS 独立相关，HR 为 1.04（CI：1.01-1.06，P = 0.003）。KEGG通路富集分析表明，重叠的DEG主要参与细胞周期。最后，我们确定了一个枢纽基因 CDK1，据报道它作用于细胞周期，与 KEGG 富集分析的结果一致。总的来说，这些数据证实 LPCAT1 在 HCC 中上调，并且是预后的独立预测因子。