Divalent copper (Cu${}^{2+}$) and oxytetracycline (OTC) are often encountered in industrial wastewater and municipal wastewater, whereas no systematic report has been found in evaluating the impacts of combined Cu${}^{2+}$ and OTC on the performance of bioreactor treatment wastewater. In the present study, the performance, nitrogen removal rate, microbial community and enzymatic activity of sequencing batch reactors (SBRs) were compared under the single and combined 20 mg/L Cu${}^{2+}$ and 5 mg/L OTC. Compared with the absence of Cu${}^{2+}$ and OTC, the COD and NH${}_4^{+}$-N removal efficiencies at 5 mg/L OTC was virtually unchanged during the whole operation process, whereas the presence of single Cu${}^{2+}$ and combined Cu${}^{2+}$ and OTC inhibited the COD and NH${}_4^{+}$-N removal. The combined Cu${}^{2+}$ and OTC had more inhibitory effects on the oxygen uptake rate, nitrification rate and denitrification rate of activated sludge than the single Cu${}^{2+}$ or OTC. The activities of dehydrogenase, ammonia monooxygenase, nitrite oxidoreductase, nitrite reductase and nitrate reductase were inhibited more by the combined Cu${}^{2+}$ and OTC than the single Cu${}^{2+}$ or OTC. The microbial reactive oxygen species production and lactate dehydrogenase release under the combined Cu${}^{2+}$ and OTC were more than those under the single Cu${}^{2+}$ or OTC under the same operational time. The microbial richness and diversity of SBRs displayed some obvious differences under the single and combined Cu${}^{2+}$ and OTC. These findings will be helpful to better understand the combined effects of heavy metals and antibiotics on biological wastewater treatment systems.

二价铜（Cu${}^{2+}$）和土霉素（OTC）在工业废水和市政废水中经常遇到，而在评估组合铜的影响方面尚未找到系统的报告${}^{2+}$以及OTC对生物反应器处理废水的性能影响。在本研究中，比较了单次和联合20 mg / L铜条件下测序间歇反应器（SBR）的性能，脱氮率，微生物群落和酶活性。${}^{2+}$和5 mg / L的OTC。与无铜相比${}^{2+}$ 以及OTC，COD和NH${}_4^{+}$在整个操作过程中，在5 mg / L OTC下的-N去除效率几乎没有变化，而存在单个Cu${}^{2+}$ 并结合铜${}^{2+}$ OTC抑制COD和NH${}_4^{+}$-N删除。组合铜${}^{2+}$ 与OTC相比，OTC对活性污泥的摄氧率，硝化率和反硝化率具有更大的抑制作用。${}^{2+}$或OTC。联合使用的Cu对脱氢酶，氨单加氧酶，亚硝酸盐氧化还原酶，亚硝酸盐还原酶和硝酸盐还原酶的抑制作用更大。${}^{2+}$ 和OTC比单铜${}^{2+}$或OTC。铜结合下微生物活性氧的产生和乳酸脱氢酶的释放${}^{2+}$ 和OTC大于单铜下的${}^{2+}$或OTC在相同的操作时间。单铜和复合铜下SBRs的微生物丰富度和多样性表现出明显差异。${}^{2+}$和场外交易。这些发现将有助于更好地了解重金属和抗生素对生物废水处理系统的综合影响。