Background

Diabetes especially type 2 diabetes mellitus (T2DM) is securing the rank under five around the world and leading cause of death cardiac disease and cancer. T2DM is a severe inflammatory state mainly associated to obesity and enhanced oxidative stress.

Objectives

We have investigated eleven genetic polymorphisms of seven important genes viz. IL-10, TNF-α, IL-6, adiponectin, IL-1, IL-18, and Vitamin D-receptor. We have focused to investigate the haplotypes, gene-gene interactions and their role in determining individual susceptibility to T2DM and their manifestation.

Methods

Eleven genetic variants of seven important candidate genes in 440 T2DM individuals and 440 controls were genotyped using conventional polymerase chain reaction (PCR) and PCR restriction fragment length polymorphism (PCR-RFLP). All calculations were done by SPSS software and LD were analysed by haploview (SHEsis software).

Results

Genotypic frequency of rs1800872, rs1800795, rs2241766, rs17846866 and rs1946518 showed highly significant association in our population. While allele frequencies of rs1800795, rs2241766 and rs1946518 showed highly significant association. The allele set of “CGGAGGTAFTB”, “CGGAGTTAFTB” and “CGGATGTCFTB” increase the chance of diabetes up to 1.6 times. This study reflects that these individuals are more susceptible of having T2DM.

Conclusions

The present study will provide a new insight in the development and the manifestation of T2DM. These genetic studies showing gene interaction for the susceptibility of the disease may be used as prognostic markers and alter treatment strategies for T2DM.

中文翻译：

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涉及2型糖尿病表现的7个重要候选基因的11个遗传变异

背景

糖尿病，尤其是2型糖尿病（T2DM），在世界范围内排名前五，是导致心脏病和癌症死亡的主要原因。T2DM是一种严重的炎症状态，主要与肥胖和氧化应激增加有关。

目标

我们已经研究了七个重要基因的十一个遗传多态性。IL-10，TNF-α，IL-6，脂联素，IL-1，IL-18和维生素D受体。我们着重研究了单倍型，基因-基因相互作用及其在确定个体对T2DM易感性及其表现中的作用。

方法

使用常规聚合酶链反应（PCR）和PCR限制性片段长度多态性（PCR-RFLP）对440个T2DM个体和440个对照中的七个重要候选基因的11个遗传变异进行基因分型。所有计算均通过SPSS软件完成，LD通过haploview（SHEsis软件）进行分析。

结果

rs1800872，rs1800795，rs2241766，rs17846866和rs1946518的基因型频率在我们的人群中显示出高度显着的关联。rs1800795，rs2241766和rs1946518的等位基因频率显示出高度显着的关联。“ CGGAGGTAFTB”，“ CGGAGTTAFTB”和“ CGGATGTCFTB”等位基因组将糖尿病的机会增加了1.6倍。这项研究表明，这些人更容易患有T2DM。

结论

本研究将为T2DM的发展和表现提供新的见解。这些遗传学研究表明该疾病易感性的基因相互作用可以用作预后标志物并改变T2DM的治疗策略。