Oxidation behavior of tretinoin (TRN) was evaluated using anodic stripping voltammetry (ASV), differential pulse voltammetry (DPV) and cyclic voltammetry (CV) techniques in Britton-Robinson (B-R) pH 7. The glassy carbon (GC) electrode was used to deposit the compound (TRN) into its surface to record a well-defined oxidation peak at 0.75 V in the presence of Ag/AgCl reference electrode and a Pt counter electrode. Some analytical parameters were studied to obtain the best oxidation signal, for example; buffer solution, pH, accumulation potential and time, amplitude, frequency, scan rate and convection rate. The high sensitivity anodic peak was obtained using B-R, pH 7, 40 s accumulation time, −0.6 V accumulation potential, 50 mV amplitude, 20 Hz frequency, 350 mV s⁻¹ scan rate, and 1000 rpm. These parameters were chosen as optimum conditions for the continued experiments. The analytical performance of the used technique was evaluated using the study of repeatability, stability, recovery, calibration curve and detection limit. Repeatability and stability was monitored for 5 × 10⁻⁶ mol L⁻¹ of TRN to give 0.24% relative standard deviation (RSD%) for eighth anodic measurements, while the stability was been very well for 80 min. The calibration curve was studied over the range of 1 × 10⁻⁶–1 × 10⁻⁵ mol L⁻¹ (n = 6), yielded, a linear relationship between concentrations and anodic current for TRN. A detection limit (LOD) was calculated to be 7.5 × 10⁻⁹ mol L⁻¹ (2.25 ppb), while a quantification limit (LOQ) was yielded 2.49 × 10⁻⁸ mol L⁻¹ (7.47 ppb). Anodic stripping voltammetry method was developed and applied for the determination of TRN in the human urine and plasma samples.

在Britton-Robinson（BR）pH 7中，使用阳极溶出伏安法（ASV），微分脉冲伏安法（DPV）和循环伏安法（CV）技术评估维甲酸（TRN）的氧化行为。使用玻璃碳（GC）电极在有Ag / AgCl参比电极和Pt对电极的情况下，将化合物（TRN）沉积到其表面以记录一个清晰的0.75 V氧化峰。研究了一些分析参数以获得最佳的氧化信号。缓冲溶液，pH，累积电位和时间，幅度，频率，扫描速率和对流速率。使用BR，pH 7、40 s累积时间，-0.6 V累积电位，50 mV振幅，20 Hz频率，350 mV s ^-1获得高灵敏度阳极峰扫描速度和1000 rpm。这些参数被选择作为连续实验的最佳条件。通过对重复性，稳定性，回收率，校正曲线和检测限的研究，评估了所用技术的分析性能。监测5×10 ^-6  mol L ^-1的TRN的重复性和稳定性，得出第八次阳极测量的相对标准偏差（RSD％）为0.24％，而稳定性在80分钟内表现得很好。在1×10 ^-6 –1×10 ^-5 mol L ^-1（n = 6）的范围内研究校准曲线，得出TRN浓度与阳极电流之间的线性关系。计算出的检出限（LOD）为7.5×10 ^-9 mol L ^-1（2.25 ppb），而定量限（LOQ）得到2.49×10 ^-8  mol L ^-1（7.47 ppb）。建立了阳极溶出伏安法，并将其用于人尿和血浆样品中TRN的测定。