Objective

This study investigated how a chemical-induced analgesia and inflammation could be managed by hot methanol extract of Leea macrophylla (Roxb.) root (LM).

Methods

Nature of secondary metabolites and the phytochemicals was studied by the established qualitative tests and GC–MS analysis. The analgesic potential was tested by acetic acid-induced writhing model, formalin test and tail immersion model. In contrast, carrageenan and histamine-induced paw edema models were applied to estimate the impact on inflammatogenic agents in Wistar albino rats.

Results

The LM was found to contain cardiac glycosides, flavonoids, tannins and triterpenoids. A list of about thirty compounds from the GC–MS study of LM has been recorded. The LM significantly (p < 0.05) minimized the writhing responses in acetic acid-induced writhing assay. The LM100 (LM 100 mg / kg b.w.) and LM200 (LM 200 mg / kg b.w.) reduced the licking period both in early and late phases of formalin-induced animal studies. The LM50 (LM 50 mg/kg b.w.) demonstrated the analgesic effects at 180 min, and in the tail immersion test diclofenac sodium displayed a substantial latency time at 120 and 150 min. The paw edema inhibition of LM100 and LM200 was statistically significant compared to diclofenac sodium of carrageenan-induced test. Also, the paw edema size in histamine-induced paw edema model was significantly decreased by LM50.

Conclusion

Results demonstrated that L. macrophylla (Roxb.) root extract could be very potential source of therapeutic formulation in pain and inflammation with further clarification.

中文翻译：

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大叶利阿（Roxb。）的甲醇热提取物可控制啮齿动物模型中的化学诱导的炎症

目的

这项研究调查了如何通过大叶李（Roxb。）根（LM）的甲醇热提取物处理化学诱导的镇痛和炎症。

方法

通过建立的定性测试和GC-MS分析研究了次生代谢产物和植物化学物质的性质。通过乙酸诱导的扭体模型，福尔马林试验和尾部浸没模型测试了止痛效果。相比之下，角叉菜胶和组胺诱导的爪水肿模型被用于估计对Wistar白化病大鼠中发炎剂的影响。

结果

发现LM含有强心苷，类黄酮，单宁和三萜。记录了来自LM的GC-MS研究的大约30种化合物的清单。LM显着（p <0.05）使乙酸诱导的扭曲试验中的扭曲反应最小化。LM100（LM 100 mg / kg bw）和LM200（LM 200 mg / kg bw）在福尔马林诱导的动物研究的早期和晚期均减少了舔食时间。LM50（LM 50 mg / kg bw）在180分钟时表现出镇痛作用，而在尾部浸入试验中，双氯芬酸钠在120和150分钟时表现出明显的潜伏时间。与角叉菜胶诱导试验的双氯芬酸钠相比，LM100和LM200的爪水肿抑制作用具有统计学意义。此外，LM50可以显着降低组胺诱导的爪水肿模型中的爪水肿大小。

结论

结果表明，大叶紫苏（Roxb。）根提取物在疼痛和炎症中可能是非常潜在的治疗制剂来源，需要进一步澄清。