The occurrence of antibiotic resistant microorganisms in poultry litter is due to the increasing use of antibiotics. Poultry litter is used as a fertilizer for its high nutrient content. The presence of antibiotic resistant microorganisms in poultry litter makes its handling, amendment and application as fertilizer a challenging task. This study is designed to evaluate the antibiotic susceptibility and plasmid profiling of beta-lactamase producing multi-drug resistant Staphylococcus aureus in poultry litter. Sample collected from a poultry farm was screened for the presence of Staphylococcus aureus strains. The in vitro antibiotic susceptibility test by disk diffusion method were carried out in all the isolates. All the isolates were highly susceptible to oxacillin and gentamycin and intermediate for erythromycin and were completely resistant to vancomycin and penicillin. The isolated Staphylococcus aureus strains showed beta-lactamase activity which is the basis for the resistance to beta-lactum antibiotics. These strains when exposed to curing agent namely ethidium bromide, the beta-lactamase activity was lost. Results indicate that Staphylococcus aureus isolated from poultry litter are beta-lactamase producers and relatively resistant to commonly used antibiotics. The resistance among the isolated strains is found to be plasmid-mediated. The outcome of our present study exercises both usefulness in shaping the existing antibiotics prescription and thus helps to reduce or prevent the development of multiple drug resistant microorganism.

家禽垫料中抗生素抗性微生物的出现是由于抗生素使用的增加。家禽垫料因其高养分含量而被用作肥料。家禽垫料中存在抗生素抗性微生物，使其作为肥料的处理，改良和应用成为一项艰巨的任务。本研究旨在评估家禽垫料中产生β-内酰胺酶的多药耐药金黄色葡萄球菌的抗生素敏感性和质粒图谱。筛选从家禽场收集的样品中是否存在金黄色葡萄球菌菌株。在体外所有分离株均采用圆盘扩散法进行药敏试验。所有分离株均对奥沙西林和庆大霉素以及红霉素中间体高度敏感，对万古霉素和青霉素完全耐药。分离出的金黄色葡萄球菌菌株显示出β-内酰胺酶活性，这是抗β-乳球抗生素的基础。这些菌株暴露于固化剂即溴化乙锭时，β-内酰胺酶活性丧失。结果表明金黄色葡萄球菌从家禽垃圾中分离出的是β-内酰胺酶生产者，对常用抗生素具有相对的抗性。发现分离菌株之间的抗性是质粒介导的。我们目前的研究结果既有助于形成现有的抗生素处方，又有助于减少或预防多种耐药微生物的发展。