Seminal plasma (SP) regulates immune responses in the female reproductive tract through specific cytokines. It is not known whether SP from high fertility bulls (H) differs from SP from low fertility bulls (L). In this study, the cytokine response of bovine endometrial epithelial cells (bEEC) in culture was investigated after challenge with SP from two bulls of below average (L) or three bulls of above average fertility (H). The bEECs were challenged with 1% or 4% SP from l- or H-fertility bulls (L1, L4, H1, H4, respectively) or 1% or 4% PBS as control (C1, C4) for 72 h. The culture media were analysed for concentrations (pg/million cells) of transforming growth factor beta (TGF-β1, TGF-β2 and TGF-β3) by Luminex, and Interleukin 6 and 8 (IL-6, IL-8) by ELISA. Challenge significantly affected production of TGF-ß1, TGF-ß2 and IL-8 compared to controls and was affected by bull fertility (p < 0.0001), SP concentration (p < 0.0001) and their interaction (p < 0.0001). A higher production of TGF-β1, TGF-β2 and IL-8 (p < 0.0001), and also IL-6 (p < 0.01), resulted from challenge with high doses of SP, being higher for L than H (p < 0.05). For TGF-β3, fertility of bull (p < 0.05). For TGF-B3, fertility of bull (p < 0.05) and the interaction between fertility and concentration of SP were significant (p < 0.01). In conclusion, 4% SP from L bulls stimulated more TGF-β1, TGF-β2, TGF-β3, IL-6 and IL-8 production than SP from H bulls, indicating that stimulation of the endometrium is relevant for fertility. Seminal plasma from high fertility bulls seems to affect cytokine production in utero positively in inseminated cows.

精浆 (SP) 通过特定细胞因子调节女性生殖道中的免疫反应。不知道来自高繁殖力公牛 (H) 的 SP 是否与来自低繁殖力公牛 (L) 的 SP 不同。在这项研究中，在用来自低于平均水平 (L) 的两头公牛或高于平均生育力 (H) 的三头公牛的 SP 攻击后，研究了培养物中牛子宫内膜上皮细胞 (bEEC) 的细胞因子反应。用 1% 或 4% 的来自l- 或 H-生育公牛（分别为 L1、L4、H1、H4）或 1% 或 4% PBS 作为对照（C1、C4）72 小时。通过 Luminex 分析培养基中转化生长因子 β（TGF-β1、TGF-β2 和 TGF-β3）的浓度（pg/百万细胞），通过 ELISA 分析白细胞介素 6 和 8（IL-6、IL-8）的浓度. 与对照相比，攻击显着影响 TGF-ß1、TGF-ß2 和 IL-8 的产生，并受公牛生育力 ( p < 0.0001 )、SP 浓度 ( p < 0.0001 ) 及其相互作用 ( p < 0.0001 ) 的影响。TGF-β1、TGF-β2 和 IL-8 ( p < 0.0001 ) 以及 IL-6 ( p < 0.01 )的更高产量是由高剂量 SP 引起的，L 的产量高于 H ( p < 0.01 ) 0.05）。对于 TGF-β3，公牛的生育力 ( p < 0.05 )。对于TGF-B3，公牛的生育力（p < 0.05）和生育力与SP浓度之间的交互作用是显着的（p < 0.01）。总之，来自 L 公牛的 4% SP 比来自 H 公牛的 SP 刺激更多的 TGF-β1、TGF-β2、TGF-β3、IL-6 和 IL-8 产生，表明子宫内膜的刺激与生育能力相关。来自高繁殖力公牛的精浆似乎对受精母牛子宫内细胞因子的产生产生积极影响。