Polar microalgae that are highly productive in cold climates can produce large amounts of biomass and polyunsaturated fatty acids (PUFA). The polar Chlamydomonas malina RCC2488, grows at low temperatures and produces high amounts of lipids, which are mainly composed of PUFA. However, not much is known about its phylogenetic relationship with other strains within the order Chlamydomonadales and the optimum growth conditions for maximum biomass productivity have not yet been identified. In this study, a phylogenetic analysis was performed to determine the closest relatives of C. malina within the Chlamydomonadales order. To select the best growth conditions for maximum biomass productivities in cultivations performed at 8 °C, different salinities (0–80) and light intensities (70–500 μmol photons m⁻² s⁻¹) were tested, using bubble column and flat-panel photobioreactors. The effect of nitrogen limitation was tested to determine if C. malina can accumulate energy reserve metabolites (carbohydrates and lipids). Phylogenetic analysis confirmed that C. malina, which belongs to the Chlamydomonales order, is closely related to the psychrophilics Chlamydomonas sp. UWO 241 and Chlamydomonas sp. SAG 75.94, as well as to the mesophilic C. parkeae MBIC 10599. The highest biomass (527 mg L⁻¹ day⁻¹), lipid (161.3 mg L⁻¹ day⁻¹) and polyunsaturated fatty acids (PUFA; 85.4 mg L⁻¹ day⁻¹) productivities were obtained at a salinity of 17.5, light intensity of 250 μmol photons m⁻² s⁻¹ and nitrogen replete conditions. Strikingly, the marine C. malina can grow even in fresh water, but the biomass productivity was reduced. While the intracellular lipid content remained unchanged under nitrogen deprivation, the carbohydrate content increased (up to 49.5% w/w), and the protein content decreased. The algal lipids were mainly comprised of neutral lipids, which were primarily composed of PUFA. Chlamydomonas malina RCC2488 is a polar marine microalga suitable for high biomass, carbohydrate, lipid and PUFA productivities at low temperatures.

在寒冷气候下高产的极性微藻类可以产生大量生物质和多不饱和脂肪酸（PUFA）。极地衣藻（Chlamydomonas malina） RCC2488在低温下生长并产生大量脂质，这些脂质主要由PUFA组成。然而，关于其与衣藻纲内其他菌株的系统发育关系还知之甚少，并且尚未确定最大生物量生产力的最佳生长条件。在这项研究中，进行了系统发育分析，以确定C. malina的近亲。在衣藻纲内。为了选择在8°C下进行的最大生物量生产的最佳生长条件，使用泡罩塔和平板色谱仪测试了不同的盐度（0–80）和光强度（70–500μmol光子m ^-2  s ^-1）。面板光生物反应器。测试了氮限制的影响，以测定小种梭状芽胞杆菌是否可以积累能量储备代谢产物（碳水化合物和脂质）。系统发育分析证实，属于衣藻纲的C. malina与嗜冷衣藻Chlamydomonas sp。UWO 241和衣藻（Chlamydomonas sp。）凹陷75.94，以及中温的C. parkeaeMBIC10599。在盐度下获得最高的生物量（527 mg L ^-1 天^-1），脂质（161.3 mg L ^-1 天^-1）和多不饱和脂肪酸（PUFA； 85.4 mg L ^-1  day ^-1）生产率。 17.5、250μmol光子m ^-2  s ^-1的光强度和氮充足的条件。令人惊讶的是，海洋C. malina甚至可以在淡水中生长，但生物量生产力下降。在氮剥夺的情况下，细胞内脂质含量保持不变，而碳水化合物含量却增加了（高达49.5％w / w），蛋白质含量降低了。藻类脂质主要由中性脂质组成，中性脂质主要由PUFA组成。衣马利纳RCC2488适合于高的生物质，碳水化合物，脂质和PUFA生产率在低温下具有极性海洋微藻。