Rhizophora mucronata Poir is widely used in traditional medicine for treatment of various diseases including cancer. Different solvent extracts (e.g., methanol, ethanol, chloroform) of R. mucronata leaves showed the presence of some bioactive components, which have been linked to antioxidant and free radical scavenging properties. This study was aimed to assess the phytochemical antioxidant active compound extracted from R. mucronata leaves and identified thought chromatographs methods. Experiments were performed with three solvent extract including methanol, chloroform and ethanol. Using qualitative and quantitative analysis of R. mucronata leave extracts and identified total phenolics, flavonoids and terpenoids, final antioxidant assay was done. Among the extracts tested, methanol extract showed very promising results in various qualitative and quantitative studies (phytochemical tests, total phenolic, total flavonoid and total terpenoid content). In addition, methanol extract exhibited high free radical scavenging activity. Hence, further studies were made for the identification of anti-oxidant active fraction from methanol extract of R. mucronata by column chromatography. Data confirmed the presence of two bands in the fraction by thin layer chromatography. The product chromatography fractions were repeatedly verified for antioxidant activity using DPPH free radical scavenging assay. Fraction 4 eluted with ethyl acetate and n-hexane combination exhibited a higher antioxidant activity in DPPH assay. From these findings, it was concluded that the methanol extract of R. mucronata leaves had maximum total phenolic, total flavonoid, total terpenoid content and maximum antioxidant activity, while its subfraction EH4 of ethyl acetate and n-hexane solvent system exhibited the highest antioxidant activity

中文翻译：

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红枣活性提取物中抗氧化组分的分离与鉴定。树叶

Rhizophora mucronata Poir 广泛用于传统医学中，用于治疗包括癌症在内的各种疾病。R. mucronata 叶子的不同溶剂提取物（例如，甲醇、乙醇、氯仿）显示存在一些生物活性成分，这些成分与抗氧化和清除自由基的特性有关。本研究旨在评估从 R. mucronata 叶中提取的植物化学抗氧化活性化合物，并确定思想色谱方法。实验采用甲醇、氯仿和乙醇三种溶剂提取物进行。使用 R. mucronata 叶提取物的定性和定量分析并确定总酚类、黄酮类和萜类化合物，完成最终的抗氧化检测。在测试的提取物中，甲醇提取物在各种定性和定量研究（植物化学测试、总酚、总黄酮和总萜类化合物含量）中显示出非常有希望的结果。此外，甲醇提取物表现出较高的自由基清除活性。因此，进一步研究了通过柱层析法鉴定红花木耳甲醇提取物中的抗氧化活性部分。数据通过薄层色谱法证实在级分中存在两条带。使用 DPPH 自由基清除试验反复验证产物色谱级分的抗氧化活性。用乙酸乙酯和正己烷组合洗脱的馏分 4 在 DPPH 测定中表现出更高的抗氧化活性。根据这些发现，可以得出结论，R. mucronata 叶的甲醇提取物的总酚含量最高，