Abstract

Characterization of selenium nanoparticles and selenium nanoparticle–human serum albumin conjugates prepared in nanopowder form, their elemental composition, and particle size distribution were investigated with a microwave plasma optical emission spectrometry operating in a single-particle mode. This new analytical technique was used for the first time to examine the molecular interaction between selenium nanoparticles and human serum albumin regarding potential biomedical applications of selenium nanoparticles. Nanopowder sample was introduced to a helium plasma by pneumatic nebulization based on fluidized bed approach and measured with a time resolution of 20 ms. Both selenium nanoparticles and selenium nanoparticle–human serum albumin conjugates were characterized by observation of synchronous signals from different particle components. Plots of the time correlation between Se and C signals for all particles in selenium nanoparticles and selenium nanoparticle–human serum albumin conjugates samples differed from each other in degree of correlation and synchronicity of recorded signals. The interaction between selenium nanoparticles and human serum albumin was confirmed using Bradford assay. For selenium nanoparticles synthesized using yeast cells, the percentage of bound protein was only of 4%, whereas for selenium nanoparticles synthesized using yeast extract as a stabilizing agent it was 16%.

Graphic abstract

中文翻译：

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使用单粒子模式的微波等离子体发射光谱法研究生物硒纳米粒子与人血清白蛋白之间的相互作用

摘要

用单粒子模式的微波等离子体发射光谱法研究了以纳米粉末形式制备的硒纳米颗粒和硒纳米颗粒-人血清白蛋白结合物的表征，其元素组成和粒径分布。这项新的分析技术首次用于检查硒纳米粒子与人血清白蛋白之间有关硒纳米粒子潜在生物医学应用的分子相互作用。通过基于流化床方法的气动雾化将纳米粉末样品引入氦等离子体，并以20毫秒的时间分辨率进行测量。硒纳米颗粒和硒纳米颗粒-人血清白蛋白结合物的特征均在于观察到来自不同颗粒成分的同步信号。硒纳米颗粒和硒纳米颗粒-人血清白蛋白结合物样品中所有颗粒的硒和碳信号之间的时间相关性图在记录信号的相关性和同步性方面互不相同。硒纳米颗粒与人血清白蛋白之间的相互作用已通过Bradford分析得以证实。对于使用酵母细胞合成的硒纳米颗粒，结合蛋白的百分比仅为4％，而对于使用酵母提取物作为稳定剂合成的硒纳米颗粒而言，其结合率为16％。硒纳米颗粒与人血清白蛋白之间的相互作用已通过Bradford分析得以证实。对于使用酵母细胞合成的硒纳米颗粒，结合蛋白的百分比仅为4％，而对于使用酵母提取物作为稳定剂合成的硒纳米颗粒而言，其结合率为16％。硒纳米颗粒与人血清白蛋白之间的相互作用已通过Bradford分析得以证实。对于使用酵母细胞合成的硒纳米颗粒，结合蛋白的百分比仅为4％，而对于使用酵母提取物作为稳定剂合成的硒纳米颗粒而言，其结合率为16％。

图形摘要