In this work, the biodegradation of by-products formed by ozonation of the azo dye Reactive Red 239 was evaluated using two MBBRs in series. Two ozone dosages were applied and low carbon removals and increasing ozone consumption observed after discoloration show the formation of oxidation resistant by-products. Five by-products were identified by GC/MS. High COD removal (90%) was observed in the biological process. However, nitrification inhibition was observed with ammonium removal of only 40%. This inhibition was probably caused by 4-amino-6-chloro-1,3,5-triazine-2-ol, which passed unscathed in the MBBRs. The nitrifying activity of the biofilm was restored when the MBBRs in series were fed with synthetic effluent (without by-products), proving the inhibition of nitrifying bacteria by ozonation by-products. The association of ozonation with the biological process was efficient in RR 239 color removal and degradation of some by-products. Higher ozone dosages are required for triazine oxidation, which probably inhibited nitrification in the MBBRs in series. The importance of identifying by-products formed by ozonation and their metabolization or not in a biological process is clear. In addition to partially inhibiting nitrification, special attention should be paid to chemicals that pass undegraded through a biological process and can be released into receiving bodies.

中文翻译：

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偶氮染料活性红 239 在串联移动床生物膜反应器中臭氧化形成的副产物的代谢

在这项工作中，使用两个串联的 MBBR 对偶氮染料活性红 239 的臭氧化形成的副产物的生物降解进行了评估。应用了两种臭氧剂量，在变色后观察到的低碳去除和臭氧消耗增加表明形成了抗氧化副产物。GC/MS 鉴定了五种副产物。在生物过程中观察到高 COD 去除率 (90%)。然而，当铵去除率仅为 40% 时观察到硝化作用抑制。这种抑制可能是由 4-amino-6-chloro-1,3,5-triazine-2-ol 引起的，它在 MBBR 中毫发无损。当串联的 MBBR 加入合成流出物（不含副产物）时，生物膜的硝化活性得以恢复，证明臭氧化副产物对硝化细菌有抑制作用。臭氧与生物过程的关联在 RR 239 颜色去除和某些副产物的降解方面是有效的。三嗪氧化需要更高的臭氧剂量，这可能会抑制串联 MBBR 中的硝化作用。识别由臭氧化形成的副产物及其在生物过程中是否代谢的重要性是显而易见的。除了部分抑制硝化作用外，还应特别注意通过生物过程未降解并可释放到接收体中的化学物质。识别由臭氧化形成的副产物及其在生物过程中是否代谢的重要性是显而易见的。除了部分抑制硝化作用外，还应特别注意通过生物过程未降解并可释放到接收体中的化学物质。识别由臭氧化形成的副产物及其在生物过程中是否代谢的重要性是显而易见的。除了部分抑制硝化作用外，还应特别注意通过生物过程未降解并可释放到接收体中的化学物质。