In microalgae biotechnology, several strategies have been used aiming to increase biomass productivity and oil content, for example, by exploring novel bioreactor design and cultivation techniques. In the present study, the influence of stressing agents (sodium thiosulphate, sodium chloride, and glycerol), under nitrogen starvation, were evaluated for lipid accumulation in Neochloris oleoabundans. Additionally, different light regimes and the use of LED were also evaluated. The results showed that sodium thiosulfate, glycerol, and sodium chloride addition resulted in a reduction in biomass concentration, but, at the same time, an increase in lipid content. 2.5 mM of sodium thiosulfate provided the highest percentage of lipid content up to 44.7%. In a tubular photobioreactor, despite the twofold increase in biomass concentration, there was a decrease in lipid content, compared with cultures in Erlenmeyer flasks. Also, the use of white LED in combination with traditional fluorescent lamp (12 h:12 h) could efficiently replace 24 h light with fluorescent lamp for producing oil-rich N. oleoabundans biomass.

中文翻译：

---

评价 Neochloris oleoabundans 作为富油生物质的可持续来源

在微藻生物技术中，已经使用了几种旨在提高生物质生产力和含油量的策略，例如，通过探索新的生物反应器设计和培养技术。在本研究中，在氮饥饿条件下，应激剂（硫代硫酸钠、氯化钠和甘油）对新绿藻中脂质积累的影响进行了评估。此外，还评估了不同的光照条件和 LED 的使用。结果表明，硫代硫酸钠、甘油和氯化钠的添加导致生物量浓度降低，但同时脂质含量增加。2.5 mM 硫代硫酸钠提供最高百分比的脂质含量，高达 44.7%。在管式光生物反应器中，尽管生物质浓度增加了两倍，与锥形瓶中的培养物相比，脂质含量有所降低。此外，将白光 LED 与传统荧光灯（12 小时：12 小时）结合使用可以有效地用荧光灯代替 24 小时的光来生产富含油的 N. oleoabundans 生物质。