Biomedical field has been seeking a feasible standard drug screening system consisting of 3D tumor model array for drug researching due to providing sufficient samples and simulating actual in vivo tumor growth situation, which is still a challenge to rapidly and uniformly establish though. Here, we propose a novel drug screening system, namely 3D tumor array chip with “layer cake” structure, for drug screening. Accurate gelatin methacryloyl hydrogel droplets (~ 0.1 μL) containing tumor cells can be automatically deposited on demand with electrohydrodynamic 3D printing. Transparent conductive membrane is introduced as a chip basement for preventing charges accumulation during fabricating and convenient observing during screening. Culturing chambers formed by stainless steel and silicon interlayer is convenient to be assembled and recycled. As this chip is compatible with the existing 96-well culturing plate, the drug screening protocols could keep the same as convention. Important properties of this chip, namely printing stability, customizability, accuracy, microenvironment, tumor functionalization, are detailly examined. As a demonstration, it is applied for screening of epirubicin and paclitaxel with breast tumor cells to confirm the compatibility of the proposed screening system with the traditional screening methods. We believe this chip will potentially play a significant role in drug evaluation in the future.

中文翻译：

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用于药物筛选的新型3D肿瘤阵列芯片的生物打印

由于提供足够的样本并模拟实际的体内肿瘤生长情况，生物医学领域一直在寻求一种可行的标准药物筛选系统，该系统包括用于药物研究的3D肿瘤模型阵列，但这仍然是快速且统一建立的挑战。在这里，我们提出了一种新颖的药物筛选系统，即具有“层状蛋糕”结构的3D肿瘤阵列芯片，用于药物筛选。包含肿瘤细胞的准确明胶甲基丙烯酰基水凝胶小滴（〜0.1μL）可通过电动3D打印自动按需沉积。引入透明导电膜作为芯片基底，以防止在制造过程中积累电荷并在筛选过程中方便观察。由不锈钢和硅中间层形成的培养室便于组装和回收。由于该芯片与现有的96孔培养板兼容，因此药物筛选方案可以与常规保持一致。详细检查了该芯片的重要特性，即打印稳定性，可定制性，准确性，微环境，肿瘤功能化。作为演示，它被用于乳腺肿瘤细胞的表柔比星和紫杉醇的筛选，以确认所提出的筛选系统与传统筛选方法的兼容性。我们相信，该芯片将来可能会在药物评估中发挥重要作用。被详细检查。作为演示，它被用于乳腺肿瘤细胞的表柔比星和紫杉醇的筛选，以确认所提出的筛选系统与传统筛选方法的兼容性。我们认为，该芯片将来可能会在药物评估中发挥重要作用。被详细检查。作为演示，它被用于乳腺肿瘤细胞的表柔比星和紫杉醇的筛选，以确认所提出的筛选系统与传统筛选方法的兼容性。我们认为，该芯片将来可能会在药物评估中发挥重要作用。