Examination of genes encoding for the virulence factors, hemolysin/cytolysin (vvhA) and capsular polysaccharide (CPS allele 1), during biofilm formation revealed that their expression was influenced by the maturity of the biofilm as well as by temperature. At 24 °C, expression of vvhA during biofilm formation was low between 4 and 12 h but increased 10-fold by 24 h to (5.1 × 10⁴ ± 6.3 × 10³mRNA copies/ml) as the biofilm matured. Compared to planktonic cells, expression of vvhA during biofilm formation at 24 °C was initially up-regulated at 4 h (1.07 ± 0.00-fold) but then was down-regulated almost four-fold during the intermediate and mature stages of biofilm formation. In contrast, vvhA expression at 37 °C was up-regulated almost four-fold in the early stages (4 and 6 h) of biofilm formation and remained two-fold up-regulated by 24 h even as the biofilm was deteriorating. CPS allele 1 expression at 24 °C during biofilm formation was up-regulated (1.50 ± 0.18-fold) during the initial attachment phase of the cells but was strongly down-regulated during the intermediate phases at 8 and 10 h (74.42 ± 42.16-fold and 453.76 ± 193.32-fold, respectively), indicating that capsular polysaccharide (CPS) is not important to intermediate biofilm architecture. Interestingly, as the biofilm matured by 24 h, expression of CPS allele 1 was again up-regulated (1.88 ± 1.07), showing that CPS plays a role in mature biofilm. At 37 °C, CPS allele 1 expression was significantly up-regulated (up to 10⁵) during biofilm formation, indicating that the biofilm form of V. vulnificus may be preferred over the planktonic form in the human host.

在生物膜形成过程中对编码毒力因子，溶血素/细胞溶素（vvhA）和荚膜多糖（CPS等位基因1）的基因进行检查后发现，它们的表达受生物膜成熟度和温度的影响。在24°C下，生物膜形成过程中vvhA的表达在4至12小时之间较低，但随着生物膜的成熟，在24小时内vvhA的表达增加了10倍，达到（5.1×10 ⁴  ±6.3×10 ³ mRNA复制/ ml）。与浮游细胞相比，在24°C的生物膜形成过程中，vvhA的表达最初在4 h时被上调（1.07±0.00倍），但随后在生物膜形成的中间和成熟阶段被下调了近四倍。相反，在37°C时，vvhA表达在生物膜形成的早期阶段（4和6 h）几乎被上调了四倍，而在24 h时，即使生物膜变差，vvhA的表达仍被上调了两倍。生物膜形成过程中，CPS等位基因1在24°C的表达在细胞初始附着阶段上调（1.50±0.18-倍），而在中间阶段的8和10 h则强烈下调（74.42±42.16-倍数和453.76±193.32倍），表明荚膜多糖（CPS）对中间生物膜结构并不重要。有趣的是，随着生物膜在24小时内成熟，CPS等位基因1的表达再次上调（1.88±1.07），表明CPS在成熟的生物膜中起作用。在37°C时，生物膜形成过程中CPS等位基因1的表达显着上调（最高10 ⁵），表明在人宿主中，创伤弧菌的生物膜形式可能优于浮游形式。