Background

Candida albicans is the most frequent pathogenic fungus in oral cavities. It adheres to dental tissues as part of dental plaques and contributes to caries formation.

Objectives

To evaluate the effect of silver diamine fluoride (SDF) on reducing C. albicans adhesion on dentine surfaces.

Methods

Flat dentine surfaces were prepared from bovine dental disks, and samples were divided into three groups. The first and second groups were pretreated for 3 min with 299 mM or 2.99 M SDF, respectively, and the third group (control) did not undergo any SDF pretreatment. All samples were washed, inoculated with C. albicans suspension onto their dentine surface, incubated at 30 °C for 6 h, and washed again to remove any nonadherent cells. The abundance of adherent cells was investigated using colorimetric and real-time polymerase chain reaction approaches. Subsequently, the morphological changes in C. albicans by pretreatment with SDF were observed under a scanning electron microscope (SEM).

Results

SDF inhibited candidal growth at concentrations as low as 2.99 µM. Dentine disks pretreated with 299 mM or 2.99 M SDF displayed significantly fewer adhered cells as compared with the control group. Upon pretreatment with SDF, SEM images showed severe morphological changes in the cellular walls, in a dose-dependent manner, suggesting a fungicidal effect of SDF against the yeast.

Conclusion

SDF should be considered for clinical applications aimed at inhibiting dental plaque caused by C. albicans, particularly in children and elderly individuals.

中文翻译：

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氟化二胺银对减少白色念珠菌对牙本质的粘附的作用。

背景

白色念珠菌是口腔中最常见的致病真菌。它作为牙菌斑的一部分粘附在牙齿组织上，并有助于龋齿的形成。

目标

评估二胺氟化银 (SDF) 对减少白色念珠菌在牙本质表面粘附的影响。

方法

由牛牙盘制备平坦的牙本质表面，并将样品分为三组。第一组和第二组分别用 299 mM 或 2.99 M SDF 预处理 3 分钟，第三组（对照组）不进行任何 SDF 预处理。清洗所有样品，将白色念珠菌悬浮液接种到它们的牙本质表面，在 30°C 下孵育 6 小时，然后再次清洗以去除任何未贴壁的细胞。使用比色法和实时聚合酶链反应方法研究贴壁细胞的丰度。随后，在扫描电子显微镜 (SEM) 下观察了 SDF 预处理后白色念珠菌的形态变化。

结果

SDF 在低至 2.99 µM 的浓度下抑制念珠菌生长。与对照组相比，用 299 mM 或 2.99 M SDF 预处理的牙本质盘显示出明显更少的粘附细胞。用 SDF 预处理后，SEM 图像显示细胞壁出现严重的形态变化，呈剂量依赖性，表明 SDF 对酵母具有杀真菌作用。

结论

应考虑将 SDF 用于旨在抑制由白色念珠菌引起的牙菌斑的临床应用，特别是在儿童和老年人中。