Carcinostatic effects of combined use of hydrogen nano-bubbles (nano-H) and platinum–povidone (PVP-–Pt) were examined. Hydrogen-dissolved medium was prepared by hydrogen-gas bubbling with a microporous gas-emittance-terminal into a medium in the absence or presence of PVP–Pt (nano-H, nano-H/PVP–Pt). Human esophagus-derived carcinoma cells KYSE70 were repressed for cell proliferation with nano-H/PVP–Pt more markedly than with nano-H, indicating the hydrogen-intensification for PVP–Pt-alone-carcinostasis. However, the intensified carcinostasis required co-administration of nano-H and PVP–Pt, and no intensified carcinostasis was shown in two-step separate administration of nano-H and PVP–Pt. Furthermore, hydrogen bubbling into PVP–Pt-containing medium achieved more appreciable carcinostasis than mere addition of PVP–Pt into nano-H-containing medium, indicating the potent interaction of hydrogen and PVP–Pt. The nano-H/PVP–Pt-administered human tongue-derived carcinoma cells HSC-4 were repressed for cell proliferation more markedly than pre-malignant human tongue-derived epitheliocytes DOK, concurrently with more abundant intracellular Pt-intake into HSC-4 cells than DOK as analyzed by ICP-MS. Thus, PVP–Pt is able to adsorb hydrogen nano-bubbles on Pt and applicable for cancer therapy by diminishing the side-effects to normal cells.

研究了氢纳米气泡（nano-H）和铂-聚维酮（PVP--Pt）组合使用的抑癌作用。在不存在或存在PVP–Pt（纳米氢，纳米H / PVP–Pt）的情况下，通过将带有微孔气体发射端的氢气鼓泡制得的氢溶解介质。纳米H / PVP-Pt抑制人食道癌细胞KYSE70的细胞增殖比纳米H抑制得更明显，这表明单独PVP-Pt癌的氢强化作用。然而，增强的癌变需要同时服用nano-H和PVP-Pt，而在Nano-H和PVP-Pt的两步分开给药中未显示出增强的癌变。此外，与仅将PVP–Pt添加到含纳米H的介质中相比，将氢气鼓泡到包含PVP–Pt的介质中可实现更大的致癌性，表明氢与PVP-Pt的有效相互作用。纳米H / PVP–Pt给药的人舌来源的癌细胞HSC-4比恶性前的人舌来源的上皮细胞DOK更明显地抑制了细胞增殖，同时更大量的细胞内Pt摄入HSC-4细胞ICP-MS分析得出的DOK比 因此，PVP–Pt能够吸附Pt上的氢纳米气泡，并通过减少对正常细胞的副作用将其应用于癌症治疗。