Exosomes transport trace amounts of (poly)phenols.,Food & Function

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Exosomes transport trace amounts of (poly)phenols.
Food & Function ( IF 5.1 ) Pub Date : 2020-07-24 , DOI: 10.1039/d0fo01824d
Anna Arola-Arnal ₁ , María-Carmen López de Las Hazas ₂ , Lisard Iglesias-Carres ₁ , Diana C Mantilla-Escalante ₂ , Manuel Suárez ₁ , Rebeca Busto ₃ , Francesco Visioli ₄ , Cinta Bladé ₁ , Alberto Dávalos ₂

Affiliation

(Poly)phenols have varied biological activities that may account for the beneficial effects of fruits and vegetables as part of a healthy diet. Although their cellular absorption and their many mechanisms of action have been partly elucidated, their transport through the systemic circulation, other than their binding to albumin, is poorly described. We aimed at determining whether (poly)phenols can be transported by extracellular vesicles. We supplemented rats with a dietary grape seed polyphenol extract (GSPE) and we quantified (poly)phenols and their metabolites at 3 and 7 h post-gavage. After quantitative LC-MS/MS analysis of circulating aglycones, and microbial-derived, or phase II-derived metabolites we recorded a quantitatively very modest transport of (poly)phenols in plasma exosomes when isolated by commercial ultracentrifugation or precipitation kits. Our data suggest that GSPE-derived (poly)phenols are minimally, if at all, transported by exosomes.

中文翻译：

外泌体运输痕量的（多）酚。

（多）酚具有多种生物活性，这可以解释健康饮食中水果和蔬菜的有益作用。尽管已经部分阐明了它们的细胞吸收及其许多作用机制，但除结合白蛋白外，还很少描述它们通过体循环的转运。我们旨在确定（多）酚是否可以被细胞外囊泡运输。我们给大鼠补充了饮食中的葡萄籽多酚提取物（GSPE），并在灌胃后3小时和7小时对（多）酚及其代谢产物进行了定量。对循环糖苷配基和微生物来源的定量LC-MS / MS分析后，或II期相衍生的代谢产物，当通过商业超速离心或沉淀试剂盒分离时，我们记录了血浆外泌体中定量（非常）适度的（多）酚运输。我们的数据表明，GSPE衍生的（多）酚很少（如果有的话）被外来体运输。

更新日期：2020-09-23

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