Photoaging is a degenerative biological process. As a kind of pluripotent stem cells, adipose-derived stem cells (ADSCs) are widely used in the treatment of photoaging. Therefore, we aimed to find an effective way to improve the antiaging ability of ADSCs. In this study, we isolated ADSCs and assessed multilineage differentiation ability and markers. Cultured ADSCs were preconditioned with astragaloside IV (ASI) at 10⁻⁷, 10⁻⁶, and 10⁻⁵ M. Cell proliferation was assessed by CCK-8 assay and cytokine secretion by enzyme-linked immunosorbent assay (ELISA). A fibroblast photoaging model was established and cocultured with normal ADSCs or ASI-treated ADSCs. Matrix metalloproteinase-1 (MMP1) and type I procollagen (PC-I) secreted by human dermal fibroblasts were measured by ELISA. The effects of ASI-treated ADSCs on skin texture, including dermal thickness, collagen content, and microvessel density, in a photoaging animal model were analyzed using H&E staining, Masson staining, and CD31 immunohistochemistry, respectively. We found that 10⁻⁶ M ASI could significantly promote cell proliferation and stimulate robust secretion of growth factors in ADSCs. Furthermore, our data showed that ASI-treated ADSCs could markedly reverse the ultraviolet B-induced decrease of PC-I secretion and increase of MMP-1 release in fibroblasts. Moreover, in photoaged skin of nude mice, ASI-treated ADSCs significantly increased dermal thickness, collagen content, and microvessel density.

中文翻译：

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Astragaloside IV 通过增强脂肪干细胞的增殖和旁分泌活性来促进抗光老化。

光老化是一种退化的生物过程。作为一种多能干细胞，脂肪干细胞（ADSCs）被广泛应用于光老化的治疗。因此，我们旨在寻找一种有效的方法来提高 ADSCs 的抗衰老能力。在这项研究中，我们分离了 ADSCs 并评估了多向分化能力和标志物。培养的 ADSC 在 10 ^-7、10 ^-6和 10 ^{-5 时}用黄芪甲苷 IV (ASI) 预处理M. 细胞增殖通过 CCK-8 测定和细胞因子分泌通过酶联免疫吸附测定 (ELISA) 进行评估。建立成纤维细胞光老化模型，并与正常 ADSCs 或 ASI 处理的 ADSCs 共培养。人真皮成纤维细胞分泌的基质金属蛋白酶-1 (MMP1) 和 I 型原胶原 (PC-I) 通过 ELISA 进行测量。分别使用 H&E 染色、Masson 染色和 CD31 免疫组织化学分析了 ASI 处理的 ADSCs 对光老化动物模型中皮肤纹理的影响，包括真皮厚度、胶原蛋白含量和微血管密度。我们发现 10 ^-6M ASI 可以显着促进细胞增殖并刺激 ADSCs 中生长因子的强烈分泌。此外，我们的数据表明，ASI 处理的 ADSC 可以显着逆转紫外线 B 诱导的成纤维细胞中 PC-I 分泌的减少和 MMP-1 释放的增加。此外，在裸鼠的光老化皮肤中，ASI 处理的 ADSC 显着增加了真皮厚度、胶原蛋白含量和微血管密度。