Schistosomiasis is considered a neglected parasitic disease. Around 280,000 people die from it annually, and more than 779 million people are at risk of getting infected. The schistosome species which infect human beings are Schistosoma mansoni, Schistosoma haematobium, Schistosoma intercalatum, Schistosoma japonicum, Schistosoma guineensis, and Schistosoma mekongi. This disease is also of veterinary significance; the most important species being Schistosoma bovis since it causes the disease in around 160 million livestock in Africa and Asia. This work was aimed at designing and developing a genus-specific loop-mediated isothermal amplification (LAMP) method for detecting the most important schistosome species affecting humans and for the species-specific detection of S. bovis. Bioinformatics tools were used for primer design, and the LAMP method was standardised for detecting the ITS-1 region from S. intercalatum, S. haematobium, S. mansoni, S. japonicum, and S. bovis DNA (generic test) and the NADH 1 gene for specifically detecting S. bovis (at different DNA concentrations). Detection limits achieved were 1 pg DNA for S. mansoni, 0.1 pg for S. haematobium, 1 pg for S. intercalatum, and 10 pg for S. bovis. No amplification for S. japonicum DNA was obtained. The LAMP designed for the amplification of S. bovis NADH-1 worked specifically for this species, and no other DNA from other schistosome species included in the study was amplified. Two highly sensitive LAMP methods for detecting different Schistosoma species important for human and veterinary health were standardised. These methods could be very useful for the diagnosis and surveillance of schistosome infections.

中文翻译：

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通过环介导的等温扩增检测血吸虫种类的分子标记。

血吸虫病被认为是一种被忽视的寄生虫病。每年约有28万人死于这种疾病，超过7.79亿人处于被感染的危险中。它感染人类的血吸虫种类有血吸虫，埃及血吸虫，血吸虫间插，血吸虫，血吸虫guineensis和血吸虫湄公。这种疾病也具有兽医学意义。最重要的物种是牛血吸虫因为它在非洲和亚洲的约1.6亿牲畜中引起了这种疾病。这项工作旨在设计和开发一种属特异性环介导的等温扩增（LAMP）方法，用于检测影响人类的最重要的血吸虫物种，并用于牛链球菌的物种特异性检测。生物信息学工具被用于引物设计，和LAMP法被标准化用于从检测ITS-1区域S.间插，血吸虫，曼氏血吸虫，日本血吸虫，和牛链球菌DNA（一般测试）和NADH 1个特异性检测牛链球菌的基因（在不同的DNA浓度下）。实现检出限为1个微克DNA曼氏血吸虫，0.1 PG为血吸虫，1微克为S.间插，和10微克为牛链球菌。没有获得日本血吸虫DNA的扩增。设计用于扩增牛链球菌NADH-1的LAMP专门针对该物种，并且未扩增该研究中包括的其他血吸虫物种的其他DNA。标准化了两种高度灵敏的LAMP方法，用于检测对人类和兽医健康至关重要的不同血吸虫物种。这些方法对于血吸虫感染的诊断和监测可能非常有用。