Abstract

Long non-coding RNA is an endogenous non-coding RNA that has currently been proved to be an important player in cancer cell biology. In the present study, we investigated the biological role of PHACTR2-AS1 in tongue squamous cell carcinoma (TSCC). PHACTR2-AS1 was preferentially localized in the cytoplasm, and was notably upregulated in TSCC tissues. High PHACTR2-AS1 was correlated with tumour differentiation, metastatic clinical features, relapse and shortened survival time. Depletion of PHACTR2-AS1 did not affect TSCC cell viability and colony formation ability, whereas substantially inhibited cell migration and invasion in vitro and lung metastasis in vivo. Mechanistically, PHACTR2-AS1 could sponge miR-137 to increase Snail expression, resulting in triggering epithelial–mesenchymal transition process, thereby promoting TSCC cell metastasis. Taken together, our data for the first time elucidate the metastasis-promoting role of PHACTR2-AS1 in TSCC, hinting a new therapeutic target for metastatic TSCC patients.

中文翻译：

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长非编码RNA PHACTR2-AS1通过调节Snail促进舌鳞状细胞癌转移。

摘要

长非编码RNA是一种内源性非编码RNA，目前已被证明是癌细胞生物学中的重要角色。在本研究中，我们调查了PHACTR2-AS1在舌鳞状细胞癌（TSCC）中的生物学作用。PHACTR2-AS1优先定位在细胞质中，并在TSCC组织中明显上调。高PHACTR2-AS1与肿瘤分化，转移的临床特征，复发和缩短的生存时间有关。PHACTR2-AS1的消耗不会影响TSCC细胞的活力和集落形成能力，而在体外和体内肺转移方面则显着抑制细胞迁移和侵袭。从机理上讲，PHACTR2-AS1可以使miR-137海绵化，从而增加Snail的表达，从而触发上皮-间质转化过程，从而促进TSCC细胞转移。两者合计，我们的数据首次阐明了PHACTR2-AS1在TSCC中的转移促进作用，暗示了转移性TSCC患者的新治疗靶点。