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Waterborne pathogen monitoring in Jaipur, India reveals potential microbial risks of urban groundwater supply
npj Clean Water ( IF 11.4 ) Pub Date : 2020-07-23 , DOI: 10.1038/s41545-020-00081-3
Aaron Bivins , Sarah Lowry , Heather M. Murphy , Mark Borchardt , Rachel Coyte , Pawan Labhasetwar , Joe Brown

The Sustainable Development Goals require that 100 mL water samples contain no culturable E. coli to classify a water supply as “safely managed” from a microbial perspective. But small volume sampling is often insufficient for detecting microbial risks. We used culture-based measures of total coliforms and E. coli along with dead-end ultrafiltration (DEUF) and droplet digital PCR (ddPCR) to assess the microbial water quality of an urban water supply in Jaipur, India. Despite the absence of culturable E. coli in 90% of the 100 mL grab samples (n = 20) during the 10-day sampling period, we detected genes associated with protozoan and bacterial pathogens (Giardia, Cryptosporidium, and enterotoxigenic E. coli) in 3 DEUF samples of groundwater (n = 9; volume 59 to 122.4 liters). Of the three groundwater samples positive for waterborne pathogens, two were associated with 100 mL grab samples that were negative for culturable E. coli. Methods with improved analytical sensitivity, such as DEUF and ddPCR, can detect evidence of pathogens in drinking water supplies and supplement conventional culture-based methods to better inform pathogen-specific risk assessment and management.



中文翻译:

印度斋浦尔的水传播病原体监测显示城市地下水供应的潜在微生物风险

可持续发展目标要求100 mL水样品中不得包含可培养的大肠杆菌,以便从微生物角度将供水分类为“安全管理”。但是,少量采样通常不足以检测微生物风险。我们使用了基于培养的大肠菌群和大肠杆菌的测量方法,以及死端超滤(DEUF)和液滴数字PCR(ddPCR)来评估印度斋浦尔城市供水的微生物水质。尽管 在10天的采样期间内100毫升抓取样品中有90%(n = 20)没有可培养的大肠杆菌,但我们检测到了与原生动物和细菌病原体(贾第鞭毛虫隐孢子和产肠毒素的大肠杆菌)在地下水3个DEUF样品(Ñ  = 9;体积59至122.4升)。在三个对水生病原体呈阳性的地下水样品中,有两个与100 mL可培养大肠杆菌呈阴性的抓取样品有关。具有提高的分析灵敏度的方法,例如DEUF和ddPCR,可以检测饮用水中病原体的证据,并补充传统的基于培养的方法,以更好地指导特定病原体的风险评估和管理。

更新日期:2020-07-24
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