Abstract Here we report the phycosynthesis of silver nanoparticles (Ag-NPs), using Codium capitatum extract, and the synthesis of fungal chitosan nanoparticles (FC-NPs), using extracted chitosan from Aspergillus niger mycelia. Then nanoconjugates from FC/Ag-NPs were produced and evaluated. The synthesized NPs had mean particles’ size diameters of 37.2, 68.4 and 79.6 nm for Ag-NPs, FC-NPs and FC/Ag-NPs, respectively. The FTIR (Fourier-transform infrared spectroscopy) analysis of synthesized NPs indicated their cross-linkage and interaction. The antibacterial activity of each type of NPs was assayed against drug resistant pathogens of Salmonella Typhimurium and Staphylococcus aureus. All NPs had powerful inhibitory effect and FC/Ag nanoconjugates had stronger activity than the other types. Scanning micrographs of FC/Ag-NPs treated S. Typhimurium elucidated vigorous alterations in cell surfaces and lethal damage to bacterial structure after 8 h of treatment. The nanoconjugates form FC-NPs and Ag-NPs had minute particle size with increasing bioactivity as antimicrobial agents to control drug resistant bacterial pathogens, which recommends their further exploration for topical applications in biomedical sectors.

中文翻译：

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藻类合成银纳米粒子和纳米真菌壳聚糖复合物对耐药细菌病原体的协同抗菌作用

摘要 在这里，我们报告了使用头顶鳕提取物合成银纳米粒子 (Ag-NPs) 以及使用从黑曲霉菌丝体中提取的壳聚糖合成真菌壳聚糖纳米粒子 (FC-NPs)。然后生产和评估来自 FC/Ag-NPs 的纳米缀合物。对于Ag-NPs、FC-NPs 和FC/Ag-NPs，合成的NPs 的平均粒径分别为37.2、68.4 和79.6 nm。合成的 NPs 的 FTIR（傅立叶变换红外光谱）分析表明它们的交联和相互作用。测定了每种类型的纳米颗粒对鼠伤寒沙门氏菌和金黄色葡萄球菌的耐药病原体的抗菌活性。所有 NPs 都具有强大的抑制作用，FC/Ag 纳米缀合物比其他类型具有更强的活性。FC/Ag-NPs 处理的 S 的扫描显微照片。Typhimurium 阐明了处理 8 小时后细胞表面的剧烈变化和对细菌结构的致命损伤。形成 FC-NPs 和 Ag-NPs 的纳米结合物具有微小的粒径，作为抗菌剂的生物活性增加，以控制耐药细菌病原体，这建议进一步探索它们在生物医学领域的局部应用。