ABSTRACT

This study revealed effect of UV-A on the vincristine accumulation and peroxidase isozymes changes in the Catharanthus roseus plantlets and relationship between them. Effect of the UV-A on the C. roseus plantlets survivor was largely because the plantlets (two weeks age) died after irradiation by UV-A more than 30 minutes. The electrophoretic patterns of peroxidase isozymes extracted from the seedlings C. Roses of the different treated 0, 10, 20 and 30 minutes, showed the changes in numbers and intensity of peroxidase isozymes bands. Using HPLC analysis the vincristine concentration extracted from the plantlets of C. Roses which were treated by UV-A revealed that the increase in vincristine concentration values were 368, 411 and 431 µg/g dry plant leaves (DPL) corresponding 10, 20 and 30 minutes respectively, compared the control 0.0 time 331 µg/g (DPL). the UV-A can be used to stimulated the biosynthesis of the metabolite in general but, this stimulation was dependent on the irradiated time exposure.

摘要

这项研究揭示了UV-A对长春花幼苗的长春新碱积累和过氧化物酶同工酶变化的影响以及它们之间的关系。UV-A对玫瑰色念珠菌幼苗存活的影响主要是因为用UV-A照射30分钟以上后，幼苗（两周龄）死亡。分别从0、10、20和30分钟处理的幼苗C.玫瑰提取的过氧化物酶同工酶的电泳图谱显示了过氧化物酶同工酶带的数量和强度的变化。使用HPLC分析从玫瑰蔷薇的幼苗中提取的长春新碱浓度UV-A处理的结果表明，长春新碱浓度值的增加分别为10、20和30分钟，分别为368、411和431 µg / g干燥植物叶片（DPL），而对照组为0.0时间331 µg / g（DPL） ）。一般而言，UV-A可以用于刺激代谢物的生物合成，但是这种刺激取决于照射时间。