Abstract

Introduction

Microbial resistance is increasing worldwide. Investigations are underway to use nanoparticles as antimicrobial agents to reduce microbial resistance.

Materials and methods

Seven human pathogenic bacteria were selected according to common hospital infection bacteria: both gram-positive and negative. In this study, imipenem and ciprofloxacin antibiotics were used separately and in combination with human serum albumin and coacervation method for the preparation of nanoparticles. Dynamic light scattering (DLS), scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FT-IR) were used to investigate the properties of nanoparticles. Antibacterial activity was determined and compared using the disk diffusion technique and minimum inhibitory concentration (MIC) was determined.

Results

The results of DLS for imipenem, ciprofloxacin and the combination of both antibiotics were 85 nm, 135 nm, and 120 nm, and for SEM were 79.9 nm, 122.2 nm, and 111 nm, respectively. All of the synthesized nanoparticles have a spherical shape. The antibacterial effect on the disk diffusion test for Acinetobacter baumannii bacteria was more noticeable in all three HSA nanoparticle compositions than other bacteria. The use of antibiotics in the form of HSA nanoparticles reduced the MIC. This value was more significant for nanoparticles carrying both ciprofloxacin and imipenem on methicillin-resistant Staphylococcus aureus and Streptococcus mutans bacteria.

Conclusion

From the results of this study, it can be concluded that the use of HSA nanoparticles carrying the aforementioned antibiotics has more antibacterial effect against the bacteria of this study, especially S. mutans, than their free form.

中文翻译：

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评价载于人血清白蛋白中的纳米抗生素亚胺培南和环丙沙星对某些抗药性致病菌的抗菌活性

摘要

介绍

全球的微生物抵抗力正在增加。使用纳米颗粒作为抗微生物剂以降低微生物抵抗力的研究正在进行中。

材料和方法

根据常见的医院感染细菌选择了7种人类致病细菌：革兰氏阳性和阴性。在这项研究中，分别使用亚胺培南和环丙沙星抗生素，并与人血清白蛋白和凝聚法联合使用以制备纳米颗粒。使用动态光散射（DLS），扫描电子显微镜（SEM）和傅里叶变换红外光谱（FT-IR）来研究纳米粒子的性能。确定抗菌活性，并使用圆盘扩散技术进行比较，并确定最小抑菌浓度（MIC）。

结果

亚胺培南，环丙沙星和两种抗生素的组合的DLS结果分别为85 nm，135 nm和120 nm，而SEM的结果分别为79.9 nm，122.2 nm和111 nm。所有合成的纳米颗粒均具有球形。在所有三种HSA纳米颗粒组合物中，鲍曼不动杆菌对圆盘扩散测试的抗菌作用均比其他细菌更明显。HSA纳米颗粒形式的抗生素的使用降低了MIC。对于在耐甲氧西林的金黄色葡萄球菌和变形链球菌细菌上同时携带环丙沙星和亚胺培南的纳米颗粒，该值更有意义。

结论

从这项研究的结果可以得出结论，使用带有上述抗生素的HSA纳米颗粒对本研究的细菌，特别是变形链球菌，比其游离形式具有更大的抗菌作用。