ABSTRACT

With the popularization of percutaneous coronary intervention technology in clinical applications, the mortality rate of acute myocardial infarction has been significantly reduced. However, ventricular remodeling following myocardial infarction (MI) has attracted extensive attention for that it can cause malignant arrhythmia, heart failure, and even death. We aimed to investigate the effects of ginsenoside Rg2 on cardiac function and myocardial fibrosis after MI and its potential mechanism. The results demonstrated that ginsenoside Rg2 improved cardiac function and inhibited collagen deposition in mice after MI. In addition, ginsenoside Rg2 reduced the levels of fibrosis-associated genes Collagen I (Col 1), Collagen III (Col 3), and alpha-smooth muscle actin (α-SMA) by activating phosphorylated AKT in angiotensin II–induced cardiac fibroblasts. Taken together, ginsenoside Rg2 improves cardiac function and attenuates cardiac fibrosis via the AKT pathway, suggesting that ginsenoside Rg2 may be a promising drug for the prevention of ventricular remodeling after MI.

Abbreviations: MI: myocardial infarction; AMI: acute myocardial infarction; LAD: left anterior descending; ECM: extracellular matrix; Col 1: collagen I; Col 3: collagen III; α-SMA: alpha-smooth muscle actin; ROS: reactive oxygen species; SOD: superoxide dismutase; GSH: glutathione; HO-1: heme oxygenase-1; WST8: water-soluble tetrazolium salt 8.

摘要

随着经皮冠状动脉介入治疗技术在临床中的普及，急性心肌梗死的死亡率已大大降低。然而，心肌梗死（MI）后的心室重塑引起广泛关注，因为它会导致恶性心律不齐，心力衰竭甚至死亡。我们旨在研究人参皂甙Rg2对MI后心功能和心肌纤维化的影响及其潜在机制。结果表明人参皂苷Rg2改善了MI后小鼠的心脏功能并抑制了胶原蛋白的沉积。此外，人参皂甙Rg2通过激活血管紧张素II诱导的心脏成纤维细胞中的磷酸化AKT，降低了纤维化相关基因胶原I（Col 1），胶原III（Col 3）和α平滑肌肌动蛋白（α-SMA）的水平。

缩写： MI：心肌梗塞；AMI：急性心肌梗塞；LAD：左前降；ECM：细胞外基质；第1列：胶原蛋白I；col 3：胶原蛋白III; α-SMA：α平滑肌肌动蛋白；ROS：活性氧；SOD：超氧化物歧化酶；GSH：谷胱甘肽；HO-1：血红素加氧酶-1；WST8：水溶性四唑盐8。