In vitro‐produced (IVP) embryos are reported to be developmentally lesser competent than in vivo‐derived (IVD) embryos and supposed to differ in the expression of genes related with glucose metabolism. So, the present study was conducted to analyse the expression pattern of GLUT 1, 5, 8 and citrate synthase (CS) in oocytes and embryos produced in vivo or in vitro in buffalo. IVD embryos were obtained from 18 superovulated buffaloes. IVP embryos were obtained from slaughterhouse‐derived oocytes subsequently subjected to in vitro fertilization and culture. Total RNA was isolated from different stages of oocytes (immature and in vitro matured) and embryos (8–16 cell to blastocysts of IVP embryos and morula to blastocysts of IVD embryos). Results demonstrated that the expression of GLUT1, GLUT 8 increased from 8 to 16 cells to blastocyst and was significantly (p < .05) higher in IVP embryos. Expression of both genes was (p < .05) higher in IVD than in IVP blastocysts; though GLUT5 transcripts were not detected at 8‐ to 16‐cell stage IVP embryos, significantly (p < .05) higher transcripts were found at morula and blastocyst stages irrespective of embryo source with significantly (p < .05) higher expression in IVD embryos compared to IVP embryos. No significant difference was observed in citrate synthase expression in embryos at morula stage irrespective of the embryo source while significantly (p < .05) higher transcript level was observed at blastocyst stage with no difference between in vivo and in vitro embryos. It can be concluded that expression of GLUTs and CS is upregulated with progression of embryonic stage and expression is higher in in vivo embryos than in vitro counter parts; thus, it can be said that in vivo‐produced embryos are metabolically superior to in vitro embryos.

中文翻译：

---

GLUT 1、5、8 和柠檬酸合酶转录本在体外产生和体内衍生的植入前胚胎中的表达模式。

据报道，体外产生的 (IVP) 胚胎在发育上不如体内衍生 (IVD) 胚胎，并且推测与葡萄糖代谢相关的基因表达不同。因此，本研究旨在分析GLUT 1、5、8和柠檬酸合酶( CS)的表达模式) 在水牛体内或体外产生的卵母细胞和胚胎中。从 18 头超数排卵的水牛获得 IVD 胚胎。IVP 胚胎来自屠宰场来源的卵母细胞，随后进行体外受精和培养。从卵母细胞（未成熟​​和体外成熟）和胚胎（8-16 个细胞到 IVP 胚胎的囊胚和桑椹胚到 IVD 胚胎的囊胚）的不同阶段分离总 RNA。结果表明，GLUT1、GLUT 8的表达从 8 个细胞增加到 16 个细胞至胚泡，并且 在 IVP 胚胎中显着升高( p < .05)。两种基因的表达 在 IVD 中均高于( p < .05) 高于 IVP 囊胚；虽然GLUT5在 8 到 16 细胞阶段的 IVP 胚胎中未检测到 转录物，在桑椹胚和囊胚阶段发现显着 ( p < .05) 更高的转录物，而与胚胎来源 无关，与 IVD 胚胎相比，IVD 胚胎中的表达显着 ( p < .05) IVP 胚胎。无论胚胎来源如何，在桑椹胚阶段未观察到柠檬酸合酶表达的显着差异，而 在囊胚阶段观察到显着 ( p < .05) 更高的转录水平，体内和体外胚胎之间没有差异。可以得出结论，GLUTs和CS 的表达随着胚胎阶段的进展而上调并且在体内胚胎中的表达高于体外对应部分；因此，它可以说是在体内-产生的胚胎在代谢上优于体外胚胎。