Rapid quantification of coliforms in ready‐to‐eat foods using lateral‐flow immunochromatographic assay,Journal of Food Safety

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Rapid quantification of coliforms in ready‐to‐eat foods using lateral‐flow immunochromatographic assay
Journal of Food Safety ( IF 1.9 ) Pub Date : 2020-07-24 , DOI: 10.1111/jfs.12835
Tatsuya Tominaga ₁

Affiliation

The degree of coliform contamination in pastries was estimated based on culturing times until positive results were obtained with the lateral‐flow immunochromatographic assay (LFIA). Coliform genera Citrobacter, Cronobacter, Enterobacter, Klebsiella, Kluyvera, Pantoea, Raoultella, and Serratia were detected in spoiled pastries, as established with next‐generation sequencing. A lateral‐flow test strip was constructed using antibodies recognizing the above genera. The culture times required for positive detection with LFIA were 0, 3, 6, and 9 hr at initial inoculation concentrations of 3.8, 2.8, 1.8, and 0.8 log₁₀ (cfu/ml), respectively. In pastries contaminated with >5.0, 3.0–5.0, 2.0–3.0 log₁₀ (cfu/g) coliform bacteria, samples became LFIA‐positive from 3, 6, and 9 hr culture, respectively. LFIA showed negative for pastries with <2.0 log₁₀ (cfu/g) coliform contamination. The quantitative category of initial coliform content before culture was predictable with 87% accuracy. This novel method can be applied to monitor food safety of other ready‐to‐eat consumables.

中文翻译：

使用侧向流免疫色谱法快速定量即食食品中的大肠菌群

根据培养时间估算糕点中大肠菌的污染程度，直到通过侧向流免疫色谱分析（LFIA）获得阳性结果为止。按照下一代测序法，在腐败的糕点中检测到大肠菌属柠檬酸杆菌，克罗诺杆菌，肠杆菌，克雷伯菌，克鲁维拉，泛菌，劳氏菌和沙雷氏菌。使用识别上述属的抗体构建侧流试纸条。在初始接种浓度分别为3.8、2.8、1.8和0.8 log ₁₀（cfu / ml）时，用LFIA进行阳性检测所需的培养时间分别为0、3、6和9小时。在受> 5.0、3.0–5.0、2.0–3.0污染的糕点中，log ₁₀（cfu / g）大肠菌，从3、6和9 hr培养开始，样品的LFIA阳性。LFIA显示大肠菌群污染小于2.0 log ₁₀（cfu / g）的糕点呈阴性。培养前大肠菌群含量的定量类别是可预测的，准确度为87％。这种新颖的方法可用于监控其他即食食品的食品安全。

更新日期：2020-07-24

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