In this study, physiological and molecular responses of sage (Salvia officinalis) to silver nanoparticles (SNPs) were studied. It is supposed that sage oxidative responses can be activated to overcome the negative effects of SNPs. Results showed the penetration of SNPs via leaf epidermis into the parenchyma cells after foliar application. A significant decrease of photosynthetic pigments and increase of cell injury indicators, the activity of enzymatic antioxidants and also the content of non-enzymatic antioxidants were observed after exposure of sage plants to 50 and 1000 mg l-1 SNPs compared to control plants. Phenolic compounds generally increased, but not in linear response to the dose level. The most abundant phenolic acid, rosmarinic acid (RA), increased more than 8-fold at 100 mg l-1 SNPs. Furthermore, the content of RA, salvianolic acid A and B was positively correlated with the activity of phenylalanine ammonia-lyase and RA synthase, but not with tyrosine aminotransferase. It could be concluded that the content of phenolic compounds increased in response to lower SNPs concentrations (50 and 100 mg l-1 ). However, the oxidative stress responses continued above these concentrations. This article is protected by copyright. All rights reserved.

中文翻译：

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银纳米颗粒污染物激活鼠尾草中的氧化应激反应和迷迭香酸积累


在这项研究中，研究了鼠尾草（Salvia officinalis）对银纳米颗粒（SNP）的生理和分子反应。据推测，鼠尾草氧化反应可以被激活，以克服 SNP 的负面影响。结果显示，叶面施用后，SNP 通过叶表皮渗透到薄壁细胞中。与对照植物相比，鼠尾草植物暴露于 50 和 1000 mg l-1 SNP 后，光合色素显着减少，细胞损伤指标、酶抗氧化剂活性以及非酶抗氧化剂含量显着增加。酚类化合物普遍增加，但与剂量水平不呈线性响应。最丰富的酚酸迷迭香酸 (RA) 在 100 mg l-1 SNP 下增加了 8 倍以上。此外，RA、丹酚酸A、B含量与苯丙氨酸解氨酶、RA合酶活性呈正相关，与酪氨酸转氨酶活性无关。可以得出结论，酚类化合物的含量随着较低的 SNP 浓度（50 和 100 mg l-1）而增加。然而，氧化应激反应在这些浓度以上仍然持续。本文受版权保护。版权所有。