Buffers of known quality for the calibration of seawater pH_T measurements are not widely or commercially available. Although there exist published compositions for the 0.04 mol kg‐H₂O⁻¹ equimolar buffer 2‐amino‐2‐hydroxymethyl‐1,3‐propanediol (TRIS)‐TRIS · H⁺ in synthetic seawater, there are no explicit procedures that describe preparing this buffer to achieve a particular pH_T with a known uncertainty. Such a procedure is described here which makes use of easily acquired laboratory equipment and techniques to produce a buffer with a pH_T within 0.006 of the published pH_T value originally assigned by DelValls and Dickson (1998), 8.094 at 25°C. Such a buffer will be suitable for the calibration of pH measurements expected to fulfil the “weather” uncertainty goal of the Global Ocean Acidification Observation Network of 0.02 in pH_T, an uncertainty goal appropriate to “identify relative spatial patterns and short‐term variation.”

中文翻译：

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在合成海水中制备2-氨基-2-羟甲基-1,3-丙二醇（TRIS）pHT缓冲液

用于校准海水pH _T测量的质量已知的缓冲液尚不广泛或无法商购。尽管存在公开的合成海水中0.04 mol kg‐H ₂ O ^-1等摩尔缓冲液2-氨基-2-羟甲基-1,3-丙二醇（TRIS）‐TRIS·H ^+的成分，但没有明确的程序描述制备本缓冲器，以实现特定的pH _Ť与已知的不确定性。本文描述了这种程序，该程序利用容易获得的实验室设备和技术生产pH _T在公布的pH _T的0.006范围内的缓冲液最初由DelValls和Dickson（1998）在25°C时指定的值为8.094。这样的缓冲液将适合校准pH值，以实现全球海洋酸化观测网络的pH _T值为0.02的“天气”不确定性目标，该不确定性目标适用于“确定相对空间格局和短期变化”。 ”