Neoporphyra haitanensis is an economically important red seaweed that inhabits upper intertidal zones. The thallus tolerates extreme fluctuating environmental stresses (e.g., surviving more than 80% water loss during low tides). To elucidate the global molecular responses relevant to this outstanding desiccation tolerance, a quantitative proteomics analysis of N. haitanensis under different desiccation treatments as well as rehydration was performed. According to the clustering of expression patterns and the functional interpretation of the 483 significantly differentially expressed proteins, a three‐stage cellular response to desiccation stress and subsequent rehydration was proposed. Stage I: at the beginning of water loss, multiple signal transduction pathways were triggered including lipid signaling, protein phosphorylation cascades, and histone acetylation controlling acetate biosynthesis to further modulate downstream hormone signaling. Protein protection by peptidyl‐prolyl isomerase and ROS scavenging systems were also immediately switched on. Stage II: with the aggravation of stress, increases in antioxidant systems, the accumulation of LEA proteins, and the temporary biosynthesis of branched starch were observed. Multiple enzymes involved in redox homeostasis, including peroxiredoxin, thioredoxin, ascorbate peroxidase, superoxide dismutase, glutathione peroxidase, and glutathione reductase, were hypothesized to function in specific cellular compartments. Stage III: when the desiccated thalli had rehydrated for 30 mins, photosynthesis and carbon fixation were recovered, and antioxidant activities and protein structure protection were maintained at a high level. This work increases the understanding of the molecular responses to environmental stresses via a proteomic approach in red seaweeds and paves the way for further functional studies and genetic engineering.

中文翻译：

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比较定量蛋白质组学揭示了潮间带海藻NEOPORPHYRA haitanensis的干燥胁迫响应

haitanensis新海藻是一种经济上重要的红海藻，栖息于潮间带上部。all虫可以承受剧烈波动的环境压力（例如，在退潮时幸存的水分流失率超过80％）。为了阐明与此出色的脱水耐受性相关的整体分子响应，对海坛猪笼草进行了定量蛋白质组学分析在不同的干燥处理以及补液条件下进行。根据表达模式的聚类和483个差异显着表达的蛋白的功能解释，提出了对脱水压力和随后补液的三阶段细胞反应。第一阶段：在失水开始时，触发了多种信号转导途径，包括脂质信号传导，蛋白质磷酸化级联反应和组蛋白乙酰化控制乙酸酯生物合成，以进一步调节下游激素信号传导。肽基脯氨酰异构酶和ROS清除系统的蛋白质保护功能也立即启动。第二阶段：随着压力的增加，抗氧化系统的增加，LEA蛋白的积累，并观察到支链淀粉的临时生物合成。据推测，涉及氧化还原稳态的多种酶包括过氧化物酶，硫氧还蛋白，抗坏血酸过氧化物酶，超氧化物歧化酶，谷胱甘肽过氧化物酶和谷胱甘肽还原酶在特定的细胞区室中起作用。第三阶段：干燥的海藻复水30分钟后，恢复了光合作用和碳固定，抗氧化活性和蛋白质结构保护保持在较高水平。这项工作通过蛋白质组学方法在红海藻中增加了对环境胁迫的分子反应的理解，为进一步的功能研究和基因工程铺平了道路。假定超氧化物歧化酶，谷胱甘肽过氧化物酶和谷胱甘肽还原酶在特定的细胞区室中起作用。第三阶段：干燥的海藻复水30分钟后，恢复了光合作用和碳固定，抗氧化活性和蛋白质结构保护保持在较高水平。这项工作通过蛋白质组学方法在红海藻中增加了对环境胁迫的分子反应的理解，为进一步的功能研究和基因工程铺平了道路。假定超氧化物歧化酶，谷胱甘肽过氧化物酶和谷胱甘肽还原酶在特定的细胞区室中起作用。第三阶段：干燥的海藻复水30分钟后，恢复了光合作用和碳固定，抗氧化活性和蛋白质结构保护保持在较高水平。这项工作通过蛋白质组学方法在红海藻中增加了对环境胁迫的分子反应的理解，为进一步的功能研究和基因工程铺平了道路。并保持较高的抗氧化活性和蛋白质结构保护。这项工作通过蛋白质组学方法在红海藻中增加了对环境胁迫的分子反应的理解，为进一步的功能研究和基因工程铺平了道路。并保持较高的抗氧化活性和蛋白质结构保护。这项工作通过蛋白质组学方法在红海藻中增加了对环境胁迫的分子反应的理解，为进一步的功能研究和基因工程铺平了道路。